Time- and Spectral-resolved two-photon imaging of healthy bladder mucosa and carcinoma in situ

被引：60

作者：

Cicchi, Riccardo ^{[1
]}

Crisci, Alfonso ^{[2
,3
]}

Cosci, Alessandro ^{[1
]}

Nesi, Gabriella ^{[3
,4
]}

Kapsokalyvas, Dimitrios ^{[1
]}

Giancane, Saverio ^{[3
,4
]}

Carini, Marco ^{[3
,4
]}

Pavone, Francesco S. ^{[1
]}

机构：

[1] LENS, European Lab Nonlinear Spect, I-50019 Sesto Fiorentino, Italy

[2] Univ Florence, Sch Med, Dept Surg, I-50100 Florence, Italy

[3] Univ Florence, Med Crit Area, I-50100 Florence, Italy

[4] Univ Florence, Div Urol, Dept Surg, I-50100 Florence, Italy

来源：

OPTICS EXPRESS | 2010年 / 18卷 / 04期

关键词：

OPTICAL COHERENCE TOMOGRAPHY; LASER-SCANNING MICROSCOPY; MULTIPHOTON MICROSCOPY; HUMAN SKIN; EX-VIVO; 2ND-HARMONIC GENERATION; FLUORESCENCE; CELLS; AUTOFLUORESCENCE; CANCER;

D O I：

10.1364/OE.18.003840

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Combined non-linear imaging techniques were used to deeply image human ex-vivo fresh biopsies of bladder as well as to discriminate between healthy bladder mucosa and carcinoma in situ. Morphological examination by two-photon excited fluorescence and second-harmonic generation has shown a good agreement with corresponding common routine histology performed on the same samples. Tumor cells appeared slightly different in shape and with a smaller cellular-to-nuclear dimension ratio with respect to corresponding normal cells. Further differences between the two tissue types were found in both spectral emission and fluorescence lifetime distribution by performing temporal-and spectral-resolved analysis of fluorescence. This method may represent a promising tool to be used in a multi-photon endoscope, in a confocal endoscope or in a spectroscopic probe for in-vivo optical diagnosis of bladder cancer. (C)2010 Optical Society of America

引用

页码：3840 / 3849

页数：10

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