Compensation for D-glutamate auxotrophy of Escherichia coli WM335 by D-amino acid aminotransferase gene and regulation of murI expression

被引:20
|
作者
Liu, LD
Yoshimura, T
Endo, K
Kishimoto, K
Fuchikami, Y
Manning, JM
Esaki, N [1 ]
Soda, K
机构
[1] Kyoto Univ, Inst Chem Res, Kyoto 611, Japan
[2] Northeastern Univ, Dept Biol, Boston, MA 02115 USA
[3] Kansai Univ, Fac Engn, Osaka 564, Japan
关键词
glutamate racemase; D-amino acid aminotransferase; murI; expression; regulation;
D O I
10.1271/bbb.62.193
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
D-Glutamate, an indispensable component of peptidoglycans of bacteria, is provided by glutamate racemase in E. coli cells. Compensation for D-glutamate auxotrophy of E. coli WM335 cells lacking the glutamate racemase gene, murI, with the D-amino acid aminotransferase gene suggests the presence of a threshold concentration for the D-glutamate required by E. coli cells, as well as a regulation system for murI expression.
引用
收藏
页码:193 / 195
页数:3
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