CAPON: A protein associated with neuronal nitric oxide synthase that regulates its interactions with PSD95

被引：298
作者：
Jaffrey, SR
Snowman, AM
Eliasson, MJL
Cohen, NA
Snyder, SH [1 ]
机构：
[1] Johns Hopkins Univ, Sch Med, Dept Neurosci, Baltimore, MD 21205 USA
[2] Johns Hopkins Univ, Sch Med, Dept Pharmacol & Mol Sci, Baltimore, MD 21205 USA
[3] Johns Hopkins Univ, Sch Med, Dept Psychiat, Baltimore, MD 21205 USA
来源：
NEURON | 1998年 / 20卷 / 01期
关键词：
D O I：
10.1016/S0896-6273(00)80439-0
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Nitric oxide (NO) produced by neuronal nitric oxide synthase (nNOS) is important for N-methyl-D-aspartate (NMDA) receptor-dependent neurotransmitter release, neurotoxicity, and cyclic GMP elevations. The coupling of NMDA receptor-mediated calcium influx and nNOS activation is postulated to be due to a physical coupling of the receptor and the enzyme by an intermediary adaptor protein, PSD95, through a unique PDZ-PDZ domain interaction between PSD95 and nNOS. Here, we report the identification of a novel nNOS-associated protein, CAPON, which is highly enriched in brain and has numerous colocalizations with nNOS. CAPON interacts with the nNOS PDZ domain through its C terminus. CAPON competes with PSD95 for interaction with nNOS, and overexpression of CAPON results in a loss of PSD95/nNOS complexes in transfected cells. CAPON may influence nNOS by regulating its ability to associate with PSD95/NMDA receptor complexes.
引用
收藏
页码：115 / 124
页数：10
相关论文
共 41 条
  • [1] ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999
  • [2] Bredt DS, 1996, P SOC EXP BIOL MED, V211, P41
  • [3] BREDT DS, 1992, J BIOL CHEM, V267, P10976
  • [4] NITRIC-OXIDE MEDIATES GLUTAMATE-LINKED ENHANCEMENT OF CGMP LEVELS IN THE CEREBELLUM
    BREDT, DS
    SNYDER, SH
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (22) : 9030 - 9033
  • [5] LOCALIZATION OF NITRIC-OXIDE SYNTHASE INDICATING A NEURAL ROLE FOR NITRIC-OXIDE
    BREDT, DS
    HWANG, PM
    SNYDER, SH
    [J]. NATURE, 1990, 347 (6295) : 768 - 770
  • [6] ISOLATION OF NITRIC-OXIDE SYNTHETASE, A CALMODULIN-REQUIRING ENZYME
    BREDT, DS
    SNYDER, SH
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (02) : 682 - 685
  • [7] Interaction of nitric oxide synthase with the postsynaptic density protein PSD-95 and alpha 1-syntrophin mediated by PDZ domains
    Brenman, JE
    Chao, DS
    Gee, SH
    McGee, AW
    Craven, SE
    Santillano, DR
    Wu, ZQ
    Huang, F
    Xia, HH
    Peters, MF
    Froehner, SC
    Bredt, DS
    [J]. CELL, 1996, 84 (05) : 757 - 767
  • [8] Brenman JE, 1996, J NEUROSCI, V16, P7407
  • [9] THE RAT-BRAIN POSTSYNAPTIC DENSITY FRACTION CONTAINS A HOMOLOG OF THE DROSOPHILA DISKS-LARGE TUMOR SUPPRESSOR PROTEIN
    CHO, KO
    HUNT, CA
    KENNEDY, MB
    [J]. NEURON, 1992, 9 (05) : 929 - 942
  • [10] Binding of the inward rectifier K+ channel Kir 2.3 to PSD-95 is regulated by protein kinase A phosphorylation
    Cohen, NA
    Brenman, JE
    Snyder, SH
    Bredt, DS
    [J]. NEURON, 1996, 17 (04) : 759 - 767
12345