Mini-FLOTAC for counting Toxoplasma gondii oocysts from cat feces - Comparison with cell counting plates

被引:4
|
作者
Djokic, Vitomir [1 ,2 ]
Blaga, Radu [3 ]
Rinaldi, Laura [4 ]
Le Roux, Delphine [3 ]
Ducry, Tamara [1 ]
Maurelli, Maria Paola [4 ]
Perret, Catherine [1 ]
Djakovic, Olgica Djurkovic [2 ]
Cringoli, Giuseppe [4 ]
Boireau, Pascal [1 ]
机构
[1] Univ Paris Est, UMR BIPAR, Lab Sante Anim Maisons Alfort, ANSES, Maisons Alfort, France
[2] Univ Belgrade, Inst Med Res, Natl Reference Lab Toxoplasmosis, Belgrade 11129, Serbia
[3] Univ Paris Est, UMR BIPAR, Ecole Natl Vet Alfort, Maisons Alfort, France
[4] Univ Naples Federico II, Dept Vet Med & Anim Prod, Naples, Italy
关键词
Toxoplasma gondii; Oocysts; Detection; Mini-FLOTAC; Kova slide; REAL-TIME PCR; RISK-FACTORS; PREGNANT-WOMEN; INFECTION; DIAGNOSIS; TRANSMISSION; ANIMALS; IDENTIFICATION; EXTRACTION; ANTIBODIES;
D O I
10.1016/j.exppara.2014.10.009
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Oocysts of Toxoplasma gondii represent one of the most common environmental contaminants causing the zoonotic infection toxoplasmosis. The aim of the present study was to compare the Mini-FLOTAC device with traditional cell counting plates (Kova Slide) for the detection of T. gondii oocysts from feline feces. Two types of experiments were performed: (i) purified oocysts were counted in different dilutions and (ii) specific pathogen free T. gondii-negative cat feces was inoculated with numbers of purified oocysts and counting was performed directly from feces. Our analysis showed a thousand times higher sensitivity of Mini-FLOTAC (5 x 10(2) oocysts) compared to Kova Slide (5 x 10(5) oocysts). Also, when compared by McNemar's test, counting of the purified oocysts showed a higher sensitivity of Mini-FLOTAC compared to Kova Slide, for a dilution of 10(3) oocysts/ml (chi(2) = 6.1; P < 0.05). A better sensitivity was also found with Mini-FLOTAC in dilutions of 10(5) and 10(4) oocysts/ml, when counted from feces (chi(2) = 4.2 and 8.1, respectively, P < 0.05). Our results show that Mini-FLOTAC is more sensitive than traditional methods of T. gondii oocysts detection and quantification is more accurate. Furthermore, Mini-FLOTAC simplicity and cost effectiveness allow it to be used with light microscopes in any laboratory or field conditions. We therefore recommend its use for regular screening. Further studies are needed to validate Mini-FLOTAC for the detection of oocysts in soil and water samples in field conditions. (C) 2014 Elsevier Inc. All rights reserved.
引用
收藏
页码:67 / 71
页数:5
相关论文
共 21 条
  • [21] A COMPARISON OF MANUAL AND ELECTRONIC COUNTING FOR TOTAL NUCLEATED CELL COUNTS ON SYNOVIAL-FLUID FROM CANINE STIFLE JOINTS
    ATILOLA, MAO
    LUMSDEN, JH
    ROOKE, F
    CANADIAN JOURNAL OF VETERINARY RESEARCH-REVUE CANADIENNE DE RECHERCHE VETERINAIRE, 1986, 50 (02): : 282 - 284