Mini-FLOTAC for counting Toxoplasma gondii oocysts from cat feces - Comparison with cell counting plates
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Djokic, Vitomir
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Univ Paris Est, UMR BIPAR, Lab Sante Anim Maisons Alfort, ANSES, Maisons Alfort, France
Univ Belgrade, Inst Med Res, Natl Reference Lab Toxoplasmosis, Belgrade 11129, SerbiaUniv Paris Est, UMR BIPAR, Lab Sante Anim Maisons Alfort, ANSES, Maisons Alfort, France
Djokic, Vitomir
[1
,2
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Blaga, Radu
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Univ Paris Est, UMR BIPAR, Ecole Natl Vet Alfort, Maisons Alfort, FranceUniv Paris Est, UMR BIPAR, Lab Sante Anim Maisons Alfort, ANSES, Maisons Alfort, France
Blaga, Radu
[3
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Rinaldi, Laura
[4
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Le Roux, Delphine
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Univ Paris Est, UMR BIPAR, Ecole Natl Vet Alfort, Maisons Alfort, FranceUniv Paris Est, UMR BIPAR, Lab Sante Anim Maisons Alfort, ANSES, Maisons Alfort, France
Le Roux, Delphine
[3
]
Ducry, Tamara
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Univ Paris Est, UMR BIPAR, Lab Sante Anim Maisons Alfort, ANSES, Maisons Alfort, FranceUniv Paris Est, UMR BIPAR, Lab Sante Anim Maisons Alfort, ANSES, Maisons Alfort, France
Ducry, Tamara
[1
]
Maurelli, Maria Paola
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Univ Naples Federico II, Dept Vet Med & Anim Prod, Naples, ItalyUniv Paris Est, UMR BIPAR, Lab Sante Anim Maisons Alfort, ANSES, Maisons Alfort, France
Maurelli, Maria Paola
[4
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Perret, Catherine
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Univ Paris Est, UMR BIPAR, Lab Sante Anim Maisons Alfort, ANSES, Maisons Alfort, FranceUniv Paris Est, UMR BIPAR, Lab Sante Anim Maisons Alfort, ANSES, Maisons Alfort, France
Perret, Catherine
[1
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Djakovic, Olgica Djurkovic
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Univ Belgrade, Inst Med Res, Natl Reference Lab Toxoplasmosis, Belgrade 11129, SerbiaUniv Paris Est, UMR BIPAR, Lab Sante Anim Maisons Alfort, ANSES, Maisons Alfort, France
Djakovic, Olgica Djurkovic
[2
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Cringoli, Giuseppe
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Univ Naples Federico II, Dept Vet Med & Anim Prod, Naples, ItalyUniv Paris Est, UMR BIPAR, Lab Sante Anim Maisons Alfort, ANSES, Maisons Alfort, France
Cringoli, Giuseppe
[4
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Boireau, Pascal
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Univ Paris Est, UMR BIPAR, Lab Sante Anim Maisons Alfort, ANSES, Maisons Alfort, FranceUniv Paris Est, UMR BIPAR, Lab Sante Anim Maisons Alfort, ANSES, Maisons Alfort, France
Boireau, Pascal
[1
]
机构:
[1] Univ Paris Est, UMR BIPAR, Lab Sante Anim Maisons Alfort, ANSES, Maisons Alfort, France
[2] Univ Belgrade, Inst Med Res, Natl Reference Lab Toxoplasmosis, Belgrade 11129, Serbia
[3] Univ Paris Est, UMR BIPAR, Ecole Natl Vet Alfort, Maisons Alfort, France
[4] Univ Naples Federico II, Dept Vet Med & Anim Prod, Naples, Italy
Oocysts of Toxoplasma gondii represent one of the most common environmental contaminants causing the zoonotic infection toxoplasmosis. The aim of the present study was to compare the Mini-FLOTAC device with traditional cell counting plates (Kova Slide) for the detection of T. gondii oocysts from feline feces. Two types of experiments were performed: (i) purified oocysts were counted in different dilutions and (ii) specific pathogen free T. gondii-negative cat feces was inoculated with numbers of purified oocysts and counting was performed directly from feces. Our analysis showed a thousand times higher sensitivity of Mini-FLOTAC (5 x 10(2) oocysts) compared to Kova Slide (5 x 10(5) oocysts). Also, when compared by McNemar's test, counting of the purified oocysts showed a higher sensitivity of Mini-FLOTAC compared to Kova Slide, for a dilution of 10(3) oocysts/ml (chi(2) = 6.1; P < 0.05). A better sensitivity was also found with Mini-FLOTAC in dilutions of 10(5) and 10(4) oocysts/ml, when counted from feces (chi(2) = 4.2 and 8.1, respectively, P < 0.05). Our results show that Mini-FLOTAC is more sensitive than traditional methods of T. gondii oocysts detection and quantification is more accurate. Furthermore, Mini-FLOTAC simplicity and cost effectiveness allow it to be used with light microscopes in any laboratory or field conditions. We therefore recommend its use for regular screening. Further studies are needed to validate Mini-FLOTAC for the detection of oocysts in soil and water samples in field conditions. (C) 2014 Elsevier Inc. All rights reserved.