Integrated magneto-fluorescence nanobeads for ultrasensitive glycoprotein detection using antibody coupled boronate-affinity recognition

被引：18

作者：

Chen, Jing ^{[1
,2
]}

Hao, Liangwen ^{[1
,2
]}

Wu, Yunqing ^{[1
,2
]}

Lin, Tong ^{[1
,2
]}

Li, Xiangmin ^{[3
]}

Leng, Yuankui ^{[1
,2
]}

Huang, Xiaolin ^{[1
,2
]}

Xiong, Yonghua ^{[1
,2
,3
]}

机构：

[1] Nanchang Univ, State Key Lab Food Sci & Technol, Nanchang 330047, Jiangxi, Peoples R China

[2] Nanchang Univ, Sch Food Sci & Technol, Nanchang 330047, Jiangxi, Peoples R China

[3] Nanchang Univ, Jiangxi OAI Joint Res Inst, Nanchang 330047, Jiangxi, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2019年 / 55卷 / 69期

基金：

中国国家自然科学基金;

关键词：

IMMUNOCHROMATOGRAPHIC ASSAY; QUANTITATIVE DETECTION; GLYCOSYLATION; OCHRATOXIN; CAPTURE;

D O I：

10.1039/c9cc04902a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Herein, we report a novel magnet-mediated antibody-boronate sandwich-typed assay (ABSTA) strategy for the ultrasensitive, specific, rapid, and enzyme-free detection of glycoproteins in complex samples. The proposed ABSTA method exhibited ultrahigh sensitivity for HCG with a detection limit of 0.19 mIU mL(-1), which is approximately 40-fold lower than that of conventional sandwich enzyme immunoassay.

引用

页码：10312 / 10315

页数：4