D-Psicose production from D-fructose using an isolated strain, Sinorhizobium sp.

被引:21
|
作者
Oh, Deok-Kun
Kim, Nam-Hee
Kim, Hye-Jung
Park, Chang-Su
Kim, Seon Won
Ko, Minsu
Park, Bueng Wan
Jung, Min Ho
Yoon, Ki-Hong
机构
[1] Woosong Univ, Sch Food Sci & Biotechnol, Taejon 300718, South Korea
[2] Konkuk Univ, Dept Biosci & Biotechnol, Seoul 143701, South Korea
[3] Gyeongsang Natl Univ, Environm Biotechnol Natl Core Res Ctr, Jinju 660701, South Korea
[4] Solgent Co Ltd, Inst Mol Genet, Taejon 305348, South Korea
关键词
biotransformation; D-fructose; isolated strain; D-Psicose; Sinorhizobium sp;
D O I
10.1007/s11274-006-9265-7
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Sinorhizobium sp., which can convert d-fructose into d-psicose, was isolated from soil. The optimal pH, temperature, and cell concentration for d-psicose production with the isolated strain were 8.5, 40 degrees C, and 60 mg/ml, respectively. The toluene-treated cells showed 2.5- and 4.8-fold increases in the d-psicose concentration and productivity compared with untreated washed cells. Under the optimal conditions, the toluene-treated cells produced 37 g d-psicose/l from 70% (w/v) (3.9 M) d-fructose after 15 h.
引用
收藏
页码:559 / 563
页数:5
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