Upconversion nanoprobes for efficiently in vitro imaging reactive oxygen species and in vivo diagnosing rheumatoid arthritis

被引：94

作者：

Chen, Zhaowei ^{[1
,2
,3
]}

Liu, Zhen ^{[1
,2
]}

Li, Zhenhua ^{[1
,2
,3
]}

Ju, Enguo ^{[1
,2
,3
]}

Gao, Nan ^{[1
,2
]}

Zhou, Li ^{[1
,2
,3
]}

Ren, Jinsong ^{[1
,2
]}

Qu, Xiaogang ^{[1
,2
]}

机构：

[1] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Rare Earth Resources Utilizat, Changchun 130022, Peoples R China

[2] Chinese Acad Sci, Changchun Inst Appl Chem, Biol Chem Lab, Changchun 130022, Peoples R China

[3] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

来源：

BIOMATERIALS | 2015年 / 39卷

基金：

中国国家自然科学基金;

关键词：

Upconversion; Nanoprobe; Hyaluronic acid; Reactive oxygen species; Rheumatoid arthritis; Bioimaging; NEAR-INFRARED-LIGHT; COLLAGEN-INDUCED ARTHRITIS; RESONANCE ENERGY-TRANSFER; ACID HYBRIDIZATION ASSAY; CORE-SHELL NANOPARTICLES; PHOTODYNAMIC THERAPY; HYALURONIC-ACID; UPCONVERTING NANOPARTICLES; CANCER-THERAPY; QUANTUM DOTS;

D O I：

10.1016/j.biomaterials.2014.10.066

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Over-generation of reactive oxygen species (ROS) is closely associated with the biological processes of rheumatoid arthritis (RA). Thus, efficient monitoring ROS in inflammatory joints would be essential for better understanding the pathogenesis and optimizing therapeutic interventions. Herein, we designed a ratiometric nanoprobe utilizing upconversion nanoparticles (UCNPs) conjugated with chromophore labeled hyaluronic acid (HA) for high sensitively sensing ROS in the aqueous solution, bioimaging ROS in inflammatory mimic cells and diagnosing RA in vivo. In this approach, the conjugation of HA conferred UCNPs not only water solubility but also biocompatibility and ROS recognizing properties. Particularly, the HA backbone cleavage and detachment of chromophore labeled HA fragments from UCNPs induced by ROS inhibited the luminescent energy transfer (LRET) and allowed rational metric upconversion luminescence (UCL) emission as the detection signal. Importantly, the upconversion nanoprobe showed high effectiveness for early assessing the treatment response of arthritic animals to an antiarthritic drug-methotrexate (MTX). (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：15 / 22

页数：8

共 59 条

[1] Toward A Multiplexed Solid-Phase Nucleic Acid Hybridization Assay Using Quantum Dots as Donors in Fluorescence Resonance Energy Transfer [J].