High-speed in vivo calcium imaging reveals neuronal network activity with near-millisecond precision

被引：378

作者：

Grewe, Benjamin F. ^{[1
]}

Langer, Dominik ^{[1
]}

Kasper, Hansjoerg ^{[1
]}

Kampa, Bjoern M. ^{[1
]}

Helmchen, Fritjof ^{[1
]}

机构：

[1] Univ Zurich, Brain Res Inst, Dept Neurophysiol, Zurich, Switzerland

来源：

NATURE METHODS | 2010年 / 7卷 / 05期

基金：

瑞士国家科学基金会;

关键词：

NEOCORTICAL PYRAMIDAL NEURONS; VISUAL-CORTEX; CELLULAR RESOLUTION; ACTION-POTENTIALS; NEURAL ACTIVITY; MICROSCOPY; AWAKE; DYNAMICS; POPULATIONS; ACTIVATION;

D O I：

10.1038/nmeth.1453

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Two-photon calcium imaging of neuronal populations enables optical recording of spiking activity in living animals, but standard laser scanners are too slow to accurately determine spike times. Here we report in vivo imaging in mouse neocortex with greatly improved temporal resolution using random-access scanning with acousto-optic deflectors. We obtained fluorescence measurements from 34-91 layer 2/3 neurons at a 180-490 Hz sampling rate. We detected single action potential-evoked calcium transients with signal-to-noise ratios of 2-5 and determined spike times with near-millisecond precision and 5-15 ms confidence intervals. An automated 'peeling' algorithm enabled reconstruction of complex spike trains from fluorescence traces up to 20-30 Hz frequency, uncovering spatiotemporal trial-to-trial variability of sensory responses in barrel cortex and visual cortex. By revealing spike sequences in neuronal populations on a fast time scale, high-speed calcium imaging will facilitate optical studies of information processing in brain microcircuits.

引用

页码：399 / U91

页数：10

共 46 条

[1] Calcium signalling: Dynamics, homeostasis and remodelling [J].

Berridge, MJ ;

Bootman, MD ;

Roderick, HL .

NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2003, 4 (07) :517-529

[2]

Borst JGG, 1998, METHOD ENZYMOL, V293, P352

[3] Spike timing-dependent plasticity: A Hebbian learning rule [J].

Caporale, Natalia ;

Dan, Yang .

ANNUAL REVIEW OF NEUROSCIENCE, 2008, 31 :25-46

[4] Spiking in primary somatosensory cortex during natural whisking in awake head-restrained rats is cell-type specific [J].

de Kock, Christiaan P. J. ;

Sakmann, Bert .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (38) :16446-16450

[5] Imaging large-scale neural activity with cellular resolution in awake, mobile mice [J].

Dombeck, Daniel A. ;

Khabbaz, Anton N. ;

Collman, Forrest ;

Adelman, Thomas L. ;

Tank, David W. .

NEURON, 2007, 56 (01) :43-57

[6] Targeted bulk-loading of fluorescent indicators for two-photon brain imaging in vivo [J].

Garaschuk, Olga ;

Milos, Ruxandra-Iulia ;

Konnerth, Arthur .

NATURE PROTOCOLS, 2006, 1 (01) :380-386

[7] Basal forebrain activation enhances cortical coding of natural scenes [J].

Goard, Michael ;

Dan, Yang .

NATURE NEUROSCIENCE, 2009, 12 (11) :1444-1449

[8] In vivo calcium Imaging of neural network function [J].

Goebel, Werner ;

Helmchen, Fritjof .

PHYSIOLOGY, 2007, 22 (06) :358-365

[9] New angles on neuronal dendrites in vivo [J].

Goebel, Werner ;

Helmchen, Fritjof .

JOURNAL OF NEUROPHYSIOLOGY, 2007, 98 (06) :3770-3779

[10] Imaging cellular network dynamics in three dimensions using fast 3D laser scanning [J].

Goebel, Werner ;

Kampa, Bjoern M. ;

Helmchen, Fritjof .

NATURE METHODS, 2007, 4 (01) :73-79

← 1 2 3 4 5 →