Acupuncture and Moxibustion Inhibited Intestinal Epithelial-Mesenchymal Transition in Patients with Crohn's Disease Induced by TGF- β 1/Smad3/Snail Pathway: A Clinical Trial Study

被引：6
作者：
Guo Sen [1 ]
Zhou Jing [2 ]
Zhang Liang [1 ]
Bao Chun-hui [3 ,4 ]
Zhao Ji-meng [3 ,4 ]
Gao Yan-ling [1 ]
Wu Pin [1 ]
Weng Zhi-jun [3 ,4 ]
Shi Yin [3 ,5 ]
机构：
[1] Shanghai Univ Tradit Chinese Med, Grad Sch, Shanghai 201203, Peoples R China
[2] Shanghai Univ Tradit Chinese Med, Shanghai Shanghai Tradit Chinese Med Integrated H, Dept Acupuncture & Moxibust, Shanghai 200082, Peoples R China
[3] Shanghai Univ Tradit Chinese Med, Dept Acupuncture & Moxibust, Yueyang Hosp Integrated Tradit Chinese & Western, Shanghai 200437, Peoples R China
[4] Shanghai Res Inst Acupuncture & Meridian, Key Lab Acupuncture & Immunol Effects, Shanghai 200030, Peoples R China
[5] Shanghai Res Inst Acupuncture & Meridian, Outpatient Dept, Shanghai 200030, Peoples R China
来源：
CHINESE JOURNAL OF INTEGRATIVE MEDICINE | 2022年 / 28卷 / 09期
基金：
中国国家自然科学基金;
关键词：
Crohn's disease; moxibustion; acupuncture; transforming growth factor beta 1/Smad3/Snail pathway; intestinal epithelial-mesenchymal transition; ACTIVATION; EXPRESSION;
D O I：
10.1007/s11655-022-2888-1
中图分类号：
R [医药、卫生];
学科分类号：
10 ;
摘要：
Objective: To explore whether acupuncture combined with moxibustion could inhibit epithelialmesenchymal transition in Crohn's disease by affecting the transforming growth factor beta 1 (TGF- beta 1)/Smad3/Snail pathway. Methods: Sixty-three patients with Crohn's disease were randomly divided into an observation group (31 cases) receiving moxibustion at 43 degrees C combined with acupuncture, and a control group (32 cases) receiving moxibustion at 37 degrees C combined with sham acupuncture using a random number table. Patients were treated for 12 weeks. Crohn's Disease Activity Index (CDAI) was used to evaluate disease activity. Hematoxylin-eosin staining and transmission electron microscopy were utilized to observe the morphological and ultrastructural changes. Immunohistochemistry was used to detect the expression of transforming growth factor beta 1 (TGF-beta 1), T beta R1, T beta R2, Smad3, Snail, E-cadherin and fibronectin in intestinal mucosal tissues. Results: The decrease of the CDAI score, morphological and ultrastructural changes were more significant in observation group. The expression levels of TGF- beta 1, T beta R2, Smad3, and Snail in the observation group were significantly lower than those before the treatment (PP<0.01). After treatment, the expression levels of TGF-beta 1, T beta R2, and Snail in the observation group were significantly lower than those in the control group (all P<0.05); compared with the control group, the expression of fibronectin in the observation group was significantly decreased, and the expression of E-cadherin was significantly increased (all P<0.05). Conclusions: Moxibustion at 43 degrees C combined with acupuncture may suppress TGF-beta 1/Smad3/Snail pathway-mediated epithelial-mesenchymal transition of intestinal epithelial cells in Crohn's disease patients by inhibiting the expression levels of TGF-beta 1, T beta R2, Smad3, and Snail.
引用
收藏
页码：823 / 832
页数：10
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