The genetic similarity of different generations of Neocallimastix frontalis SK

被引：16
作者：
Chen, YC [1 ]
Hseu, RS
Cheng, KJ
机构：
[1] Acad Sinica, Inst BioAgr Sci, Taipei 105, Taiwan
[2] Natl Taiwan Univ, Dept Agr Chem, Taipei 106, Taiwan
来源：
FEMS MICROBIOLOGY LETTERS | 2003年 / 221卷 / 02期
关键词：
anaerobic rumen fungus; genetic similarity; RAPD profiling; Neocallimastix frontalis SK;
D O I：
10.1016/S0378-1097(03)00207-6
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The genetic similarity of different generations of Neocallimastix frontalis SK was examined by random amplified polymorphic DNA (RAPD) profiling and internal transcribed spacer 1 (ITSI) sequence analysis. N. frontalis SK was subcultured every 2-4 days, and SK-1, SK-3M. and SK-1Y represented N. frontalis SK cultures after one subculture, 50 subcultures, and 150 subcultures. The DNA polymorphisms of the different N. frontalis SK generations were compared by RAPD profiling. The RAPD results gave the same patterns for SK-1. SK-3M and SK-1Y using 12 selected random primers. The partial 18S rDNA, 5.8S rDNA, and ITSI regions of different generations of X frontalis SK were amplified and sequenced. The results of alignment and pairwise similarity indicated that the analyzed rRNA sequences of SK-1, SK-3M and SK-1Y were totally identical. This study thus demonstrated genetically identical DNA polymorphisms by RAPD profiling and an unvaried ITSI region for N. frontalis SK when the strain is subcultured frequently. This suggests that this strain is homokaryotic and grows via an asexual life cycle in vitro. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
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页码：227 / 231
页数：5
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