Molecular and functional characterization of HtrA protein in Actinobacillus pleuropneumoniae

被引：6

作者：

Xu, Kui ^{[1
]}

Zhao, Qin ^{[1
]}

Jiang, Hong-Ze ^{[1
]}

Mou, Xin-Ran ^{[1
]}

Chang, Yung-Fu ^{[2
]}

Cao, Yu-Qin ^{[1
]}

Miao, Chang ^{[1
]}

Wu, Rui ^{[1
]}

Wen, Yi-Ping ^{[1
]}

Huang, Xiao-Bo ^{[1
]}

Yan, Qi-Gui ^{[1
]}

Du, Sen-Yan ^{[1
]}

Cao, San-Jie ^{[1
]}

机构：

[1] Sichuan Agr Univ, Coll Vet Med, Res Ctr Swine Dis, Chengdu, Peoples R China

[2] Cornell Univ, Coll Vet Med, Dept Populat Med & Diagnost Sci, Ithaca, NY 14853 USA

来源：

VETERINARY MICROBIOLOGY | 2021年 / 257卷

关键词：

Actinobacillus pleuropneumoniae; HtrA; Chaperone and proteolytic activities; Immune protection; ESCHERICHIA-COLI; PATHOGENESIS; VIRULENCE; SURFACE; FAMILY; HSP90; CELLS; MICE;

D O I：

10.1016/j.vetmic.2021.109058

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Actinobacillus pleuropneumoniae (A.pleuropneumoniae) causes serious economic loss for the swine industry. A hightemperature requirements A (HtrA)-like protease and its homologs have been reported to be involved in protein quality control and expression of important immunoprotective antigens in many pathogens. In this study, we showed that HtrA of A.pleuropneumoniae exhibited both chaperone and proteolytic activities. Moreover, Outer membrane protein P5 (OmpP5) in A.pleuropneumoniae and Heat shock protein 90 (Hsp90) in porcine lung tissues were first discovered and identified as specific proteolytic substrates for rHtrA. The maximum cleavage activity occurs at 50 celcius in a time-dependent manner. In addition, rHtrA mainly induced IgG 2a subtype of IgG and Th1 (IFN-gamma, IL-2) response in a mice model, and promoted a significant proliferation of spleen lymphocytes compare with negative control (P < 0.05). The survival rates of 37.5 % were observed against A.pleuropneumoniae strain. Together, these data demonstrate that rHtrA plays a multi-functional role in A.pleuropneumoniae.

引用

页数：8

共 31 条

[1] Mice immune responses to Brucella abortus heat shock proteins -: Use of baculovirus recombinant-expressing whole insect cells, purified Brucella abortus recombinant proteins, and a vaccinia virus recombinant as immunogens
Bae, JE
Schurig, GG
Toth, TE
[J]. VETERINARY MICROBIOLOGY, 2002, 88 (02) : 189 - 202
[2] Rapid paracellular transmigration of Campylobacter jejuni across polarized epithelial cells without affecting TER: role of proteolytic-active HtrA cleaving E-cadherin but not fibronectin
Boehm, Manja
Hoy, Benjamin
Rohde, Manfred
Tegtmeyer, Nicole
Baek, Kristoffer T.
Oyarzabal, Omar A.
Brondsted, Lone
Wessler, Silja
Backert, Steffen
[J]. GUT PATHOGENS, 2012, 4
[3] Actinobacillus pleuropneumoniae:: pathobiology and pathogenesis of infection
Bossé, JT
Janson, H
Sheehan, BJ
Beddek, AJ
Rycroft, AN
Kroll, JS
Langford, PR
[J]. MICROBES AND INFECTION, 2002, 4 (02) : 225 - 235
[4] Design principles of the proteolytic cascade governing the σE-mediated envelope stress response in Escherichia coli:: keys to graded, buffered, and rapid signal transduction
Chaba, Rachna
Grigorova, Irina L.
Flynn, Julia M.
Baker, Tania A.
Gross, Carol A.
[J]. GENES & DEVELOPMENT, 2007, 21 (01) : 124 - 136
[5] The HtrA protease of Borrelia burgdorferi degrades outer membrane protein BmpD and chemotaxis phosphatase CheX
Coleman, James L.
Crowley, Jameson T.
Toledo, Alvaro M.
Benach, Jorge L.
[J]. MOLECULAR MICROBIOLOGY, 2013, 88 (03) : 619 - 633
[6] Submerged cultivation of Ganoderma lucidum and the effects of its polysaccharides on the production of human cytokines TNF-α, IL-12, IFN-γ, IL-2, IL-4, IL-10 and IL-17
Habijanic, Jozica
Berovic, Marin
Boh, Bojana
Plankl, Mojca
Wraber, Branka
[J]. NEW BIOTECHNOLOGY, 2015, 32 (01) : 85 - 95
[7] Actinobacillus pleuropneumoniae infections in pigs: the role of virulence factors in pathogenesis and protection
Haesebrouck, F
Chiers, K
Van Overbeke, I
Ducatelle, R
[J]. VETERINARY MICROBIOLOGY, 1997, 58 (2-4) : 239 - 249
[8] Architecture and regulation of HtrA-family proteins involved in protein quality control and stress response
Hansen, Guido
Hilgenfeld, Rolf
[J]. CELLULAR AND MOLECULAR LIFE SCIENCES, 2013, 70 (05) : 761 - 775
[9] Hao Wu, 2010, IDENTIFICATION FUNCT
[10] Proteases in bacterial pathogenesis
Ingmer, Hanne
Brondsted, Lone
[J]. RESEARCH IN MICROBIOLOGY, 2009, 160 (09) : 704 - 710

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