Thermostable Kunitz trypsin inhibitor with cytokine inducing, antitumor and HIV-1 reverse transcriptase inhibitory activities from Korean large black soybeans

被引:51
作者
Fang, Evandro Fei [1 ]
Wong, Jack Ho [1 ]
Ng, Tzi Bun [1 ]
机构
[1] Chinese Univ Hong Kong, Fac Med, Sch Biomed Sci, Shatin, Hong Kong, Peoples R China
关键词
Korean large black soybean; Glycine max; Seeds; Purification; Trypsin inhibitor; Biological properties; AMINO-ACID-SEQUENCES; SOY ISOFLAVONES; BINDING LECTIN; PURIFICATION; SEEDS; ANTIFUNGAL; ACTIVATION;
D O I
10.1016/j.jbiosc.2009.08.483
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A large number of trypsin inhibitors belonging to various types have been purified from different kinds of legumes. In this study, by using liquid chromatography, a Kunitz type trypsin inhibitor (KBTI) with a molecular weight of 20107.645 Da was purified from Korean large black soybeans. KBTI reduced the proteolytic activities of trypsin and a-chymotrypsin with the activity of similar to 8520 BAEE units/mg and similar to 24 BTEE units/mg, respectively. It showed high thermal stability (0-100 degrees C) as well as stability over a large range of pH values (pH 3-11). Furthermore, KBTI inhibited HIV-1 reverse transcriptase activity with an IC50 value of 0.71 mu M and induced the release of pro-inflammatory cytokines such as TNF-alpha, IL-1 beta, IL-2 and interferon-gamma at the mRNA level. KBTI exerted weak antiproliferative activity toward CNE-2 and HNE-2 nasopharyngeal cancer cells, MCF-7 breast cancer cells, and Hep G2 hepatoma cells. KBTI was destitute of mitogenic, ribonuclease and antifungal activities.(C) 2009, The Society for Biotechnology, Japan. All rights reserved.
引用
收藏
页码:211 / 217
页数:7
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