Characterization of CRISPR-Cas systems in Bifidobacterium breve

被引：2

作者：

Han, Xiao ^{[1
,2
]}

Zhou, Xingya ^{[1
,2
]}

Pei, Zhangming ^{[1
,2
]}

Stanton, Catherine ^{[3
,4
,5
]}

Ross, R. Paul ^{[3
,4
]}

Zhao, Jianxin ^{[1
,2
,6
]}

Zhang, Hao ^{[1
,2
,6
,7
,8
]}

Yang, Bo ^{[1
,2
,3
]}

Chen, Wei ^{[1
,2
,6
]}

机构：

[1] Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi, Jiangsu, Peoples R China

[2] Jiangnan Univ, Sch Food Sci & Technol, Wuxi, Jiangsu, Peoples R China

[3] Jiangnan Univ, Int Joint Res Lab Pharmabiot & Antibiot Resistanc, Wuxi, Jiangsu, Peoples R China

[4] Univ Coll Cork, APC Microbiome Ireland, Cork, Ireland

[5] Teagasc Food Res Ctr, Moorepk, Cork, Ireland

[6] Jiangnan Univ, Natl Engn Res Ctr Funct Food, Wuxi, Jiangsu, Peoples R China

[7] Wuxi Translat Med Res Ctr, Wuxi, Jiangsu, Peoples R China

[8] Jiangsu Translat Med Res Inst, Wuxi Branch, Wuxi, Jiangsu, Peoples R China

来源：

MICROBIAL GENOMICS | 2022年 / 8卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Bifidobacterium breve; CRISPR-Cas system; genotyping; prophage; SPACER ACQUISITION; DOUBLE-BLIND; SUPPLEMENTATION; CLASSIFICATION; CHILDREN; BACTERIA; INFANTS; VERSION; GENOME; GUT;

D O I：

10.1099/mgen.0.000812

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The clustered regularly interspaced short palindromic repeat (CRISPR)-CRISPR-associated protein (Cas) system is an important adaptive immune system for bacteria to resist foreign DNA infection, which has been widely used in genotyping and gene editing. To provide a theoretical basis for the application of the CRISPR-Cas system in Bifidobacterium breve, the occurrence and diversity of CRISPR-Cas systems were analysed in 150 B. breve strains. Specifically, 47% (71/150) of B. breve genomes possessed the CRISPR-Cas system, and type I-C CRISPR-Cas system was the most widely distributed among those strains. The spacer sequences present in B. breve can be used as a genotyping marker. Additionally, the phage assembly-related proteins were important targets of the type I-C CRISPR-Cas system in B. breve, and the protospacer adjacent motif sequences were further characterized in B. breve type I-C system as 5'-TTC-3'. All these results might provide a molecular basis for the development of endogenous genome editing tools in B. breve.

引用

页数：12

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