L-glutamine efficiently stimulates biosynthesis of bacillomycin D in Bacillus subtilis fmbJ

被引：12
|
作者：
Qian, Shiquan [1 ,2 ]
Sun, Jing [1 ]
Lu, Hedong [1 ]
Lu, Fengxia [1 ]
Bie, Xiaomei [1 ]
Lu, Zhaoxin [1 ]
机构：
[1] Nanjing Agr Univ, Coll Food Sci & Technol, 1 Weigang, Nanjing 210095, Jiangsu, Peoples R China
[2] Bengbu Univ, Coll Food & Bioengn, Bengbu 233030, Peoples R China
来源：
PROCESS BIOCHEMISTRY | 2017年 / 58卷
基金：
中国国家自然科学基金;
关键词：
Amino acids; Bacillomycin D production; Fed-batch fermentation; Gene expression; SIGMA-FACTOR; ITURIN; ACID; ABRB; ANTIBIOTICS; LIPOPEPTIDE; INHIBITION; INITIATION; REGULATOR; CELLS;
D O I：
10.1016/j.procbio.2017.04.026
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The present study investigated the stimulation effect of L-glutamine (L-gln) on bacillomycin D biosynthesis of Bacillus subtilis fmbJ and the transcription of the synthetase genes and the signal proteins that related to the biosynthesis of bacillomycin D. It was found that L-gln significantly increased the production of bacillomycin D, in comparison with other amino acids. L-gln significantly enhanced the expression level of bmyA (BYA), bmyB (BYB), bmyC (BYC), the thioesterase gene (TE) and up-regulated DegU, o(H) and SpoOA, but down-regulated AbrB, therefore the yield of bacillomycin D significantly increased. Furthermore, when conjointly feeding 5 g/L of L-gin and 30 g/L of inulin, the production of bacillomycin D was up to 1.93 g/L.
引用
收藏
页码：224 / 229
页数：6
相关论文
共 17 条
  • [1] Effect of inulin on efficient production and regulatory biosynthesis of bacillomycin D in Bacillus subtilis fmbJ
    Qian, Shiquan
    Lu, Hedong
    Meng, Panpan
    Zhang, Chong
    Lv, Fengxia
    Bie, Xiaomei
    Lu, Zhaoxin
    BIORESOURCE TECHNOLOGY, 2015, 179 : 260 - 267
  • [2] The efficient synthesis strategy of bacillomycin D in Bacillus amyloliquefaciens fmbJ
    Lv, Ziyan
    Li, Ruili
    Shi, Changzheng
    Lu, Zhaoxin
    Meng, Fanqiang
    Bie, Xiaomei
    PROCESS BIOCHEMISTRY, 2024, 145 : 131 - 138
  • [3] Enhanced bacillomycin D yield by cyclic fermentation with diatomaceous earth immobilized Bacillus amyloliquefaciens fmbJ
    Luo, Xiaojiao
    Zhang, Jin
    Dai, Yongjin
    Lu, Zhaoxin
    Sun, Jing
    Lu, Yingjian
    PROCESS BIOCHEMISTRY, 2023, 132 : 278 - 288
  • [4] Knockout of rapC Improves the Bacillomycin D Yield Based on De Novo Genome Sequencing of Bacillus amyloliquefaciens fmbJ
    Sun, Jing
    Qian, Shiquan
    Lu, Jing
    Liu, Yanan
    Lu, Fengxia
    Bie, Xiaomei
    Lu, Zhaoxin
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2018, 66 (17) : 4422 - 4430
  • [5] Promoted Spore Formation of Bacillus amyloliquefaciens fmbJ by its Secondary Metabolite Bacillomycin D Coordinated with Mn2+
    Zhang, Jin
    Luo, Xiaojiao
    Pang, Xinyi
    Li, Xiangfei
    Lu, Yingjian
    Sun, Jing
    INDIAN JOURNAL OF MICROBIOLOGY, 2022, 62 (04) : 531 - 539
  • [6] Calcium lactate efficiently induces production of bacillomycin D in Bacillus subtilis NS-174 and inhibition of spore germination of Aspergillus flavus in rice
    Qian, Shiquan
    Li, Jingwen
    Xu, Tiansheng
    Diao, Enjie
    Tang, Yuting
    Zhou, Xinrong
    Liu, Ying
    INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, 2023, 58 (02) : 824 - 830
  • [7] L-glutamine stimulates intestinal cell proliferation and activates mitogen-activated protein kinases
    Rhoads, JM
    Argenzio, RA
    Chen, WN
    Rippe, RA
    Westwick, JK
    Cox, AD
    Berschneider, HM
    Brenner, DA
    AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 1997, 272 (05): : G943 - G953
  • [8] Anti-Candida effect of bacillomycin D-like lipopeptides from Bacillus subtilis B38
    Tabbene, Olfa
    Kalai, Leila
    Slimene, Imen Ben
    Karkouch, Ines
    Elkahoui, Salem
    Gharbi, Abdelhamid
    Cosette, Pascal
    Mangoni, Maria-Luisa
    Jouenne, Thierry
    Limam, Ferid
    FEMS MICROBIOLOGY LETTERS, 2011, 316 (02) : 108 - 114
  • [9] Bacillomycin L and surfactin contribute synergistically to the phenotypic features of Bacillus subtilis 916 and the biocontrol of rice sheath blight induced by Rhizoctonia solani
    Luo, Chuping
    Zhou, Huafei
    Zou, Jincheng
    Wang, Xiaoyu
    Zhang, Rongsheng
    Xiang, Yaping
    Chen, Zhiyi
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2015, 99 (04) : 1897 - 1910
  • [10] Genetic engineering of hexsosamine with L-glutamine D-fructose-6-phosphate amidotransferase genes in plants
    Shibatani, Shigeo
    Kitazawa, Hiroaki
    PLANT BIOTECHNOLOGY, 2009, 26 (01) : 149 - 152
12