ZipA and FtsA* stabilize FtsZ-GDP miniring structures

被引：15

作者：

Chen, Yaodong ^{[1
,2
]}

Huang, Haiyan ^{[1
]}

Osawa, Masaki ^{[2
]}

Erickson, Harold P. ^{[2
]}

机构：

[1] Northwest Univ Xian, Coll Life Sci, Minist Educ, Key Lab Resources Biol & Biotechnol Western Chin, Xian 710069, Shaanxi Provinc, Peoples R China

[2] Duke Univ, Med Ctr, Dept Cell Biol, Durham, NC USA

来源：

SCIENTIFIC REPORTS | 2017年 / 7卷

关键词：

CELL-DIVISION; BACTERIAL CYTOKINESIS; ESCHERICHIA-COLI; Z-RING; TUBULIN; DYNAMICS; CYTOSKELETON; MEMBRANE; STRAIGHT; EXCHANGE;

D O I：

10.1038/s41598-017-03983-4

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The cytokinetic division ring of Escherichia coli comprises filaments of FtsZ tethered to the membrane by FtsA and ZipA. Previous results suggested that ZipA is a Z-ring stabilizer, since in vitro experiments it is shown that ZipA enhanced FtsZ assembly and caused the filaments to bundles. However, this function of ZipA has been challenged by recent studies. First, ZipA-induced FtsZ bundling was not significant at pH greater than 7. Second, some FtsA mutants, such as FtsA* were able to bypass the need of ZipA. We reinvestigated the interaction of FtsZ with ZipA in vitro. We found that ZipA not only stabilized and bundled straight filaments of FtsZ-GTP, but also stabilized the highly curved filaments and miniring structures formed by FtsZ-GDP. FtsA* had a similar stabilization of FtsZ-GDP minirings. Our results suggest that ZipA and FtsA* may contribute to constriction by stabilizing this miniring conformation.

引用

页数：9

共 34 条

[1] Adenine Nucleotide-dependent Regulation of Assembly of Bacterial Tubulin-like FtsZ by a Hypermorph of Bacterial Actin-like FtsA [J].

Beuria, Tushar K. ;

Mullapudi, Srinivas ;

Mileykovskaya, Eugenia ;

Sadasivam, Mahalakshmi ;

Dowhan, William ;

Margolin, William .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2009, 284 (21) :14079-14086

[2] Treadmilling by FtsZ filaments drives peptidoglycan synthesis and bacterial cell division [J].

Bisson-Filho, Alexandre W. ;

Hsu, Yen-Pang ;

Squyres, Georgia R. ;

Kuru, Erkin ;

Wu, Fabai ;

Jukes, Calum ;

Sun, Yingjie ;

Dekker, Cees ;

Holden, Seamus ;

VanNieuwenhze, Michael S. ;

Brun, Yves V. ;

Garner, Ethan C. .

SCIENCE, 2017, 355 (6326) :739-743

[3] SulA Inhibits Assembly of FtsZ by a Simple Sequestration Mechanism [J].

Chen, Yaodong ;

Milam, Sara L. ;

Erickson, Harold P. .

BIOCHEMISTRY, 2012, 51 (14) :3100-3109

[4] Conformational Changes of FtsZ Reported by Tryptophan Mutants [J].

Chen, Yaodong ;

Erickson, Harold P. .

BIOCHEMISTRY, 2011, 50 (21) :4675-4684

[5] FtsZ Filament Dynamics at Steady State: Subunit Exchange with and without Nucleotide Hydrolysis [J].

Chen, Yaodong ;

Erickson, Harold P. .

BIOCHEMISTRY, 2009, 48 (28) :6664-6673

[6] Rapid in vitro assembly dynamics and subunit turnover of FtsZ demonstrated by fluorescence resonance energy transfer [J].

Chen, YD ;

Erickson, HP .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (23) :22549-22554

[7] A rapid fluorescence assay for FtsZ assembly indicates cooperative assembly with a dimer nucleus [J].

Chen, YD ;

Bjornson, K ;

Redick, SD ;

Erickson, HP .

BIOPHYSICAL JOURNAL, 2005, 88 (01) :505-514

[8] Defining the rate-limiting processes of bacterial cytokinesis [J].

Coltharp, Carla ;

Buss, Jackson ;

Plumer, Trevor M. ;

Xiao, Jie .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2016, 113 (08) :E1044-E1053

[9] Dominant C-terminal deletions of FtsZ that affect its ability to localize in Caulobacter and its interaction with FtsA [J].

Din, N ;

Quardokus, EM ;

Sackett, MJ ;

Brun, YV .

MOLECULAR MICROBIOLOGY, 1998, 27 (05) :1051-1063

[10] The discovery of the prokaryotic cytoskeleton: 25th anniversary [J].

Erickson, Harold P. .

MOLECULAR BIOLOGY OF THE CELL, 2017, 28 (03) :357-358

← 1 2 3 4 →