Overexpression and increased DNA topoisomerase II-like enzyme activity in arsenite resistant Leishmania donovani

被引：8
|
作者：
Jayanarayan, KG [1 ]
Dey, CS [1 ]
机构：
[1] Natl Inst Pharmaceut Educ & Res, Dept Biotechnol, Punjab 160062, India
来源：
MICROBIOLOGICAL RESEARCH | 2003年 / 158卷 / 01期
关键词：
topoisomerase II; Leishmania donovani; sodium arsenite resistance;
D O I：
10.1078/0944-5013-00177
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Western immunoblot analyses of whole cell lysates probed with a human specific monoclonal anti-topoisomerase Ilalpha antibody identified a 190 kDa protein over expressed in the arsenite resistant Leishmania donovani strain. The crude nuclear extract of the resistant strain showed higher topoisomerase II-like enzyme activity, suggesting a possible regulatory role of putative topoisomerase II in arsenite resistant Leishmania.
引用
收藏
页码：55 / 58
页数：4
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