Addition of Adipose-Derived Stem Cells to Mesenchymal Stem Cell Sheets Improves Bone Formation at an Ectopic Site
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作者:
Wang, Zhifa
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Fourth Mil Med Univ, Sch Stomatol, Dept Oral & Maxillofacial Surg, State Key Lab Mil Stomatol, Xian 710032, Peoples R ChinaFourth Mil Med Univ, Sch Stomatol, Dept Oral & Maxillofacial Surg, State Key Lab Mil Stomatol, Xian 710032, Peoples R China
Wang, Zhifa
[1
]
Li, Zhijin
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Fourth Mil Med Univ, Sch Stomatol, Dept Oral & Maxillofacial Surg, State Key Lab Mil Stomatol, Xian 710032, Peoples R ChinaFourth Mil Med Univ, Sch Stomatol, Dept Oral & Maxillofacial Surg, State Key Lab Mil Stomatol, Xian 710032, Peoples R China
Li, Zhijin
[1
]
Dai, Taiqiang
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Fourth Mil Med Univ, Sch Stomatol, Dept Oral & Maxillofacial Surg, State Key Lab Mil Stomatol, Xian 710032, Peoples R ChinaFourth Mil Med Univ, Sch Stomatol, Dept Oral & Maxillofacial Surg, State Key Lab Mil Stomatol, Xian 710032, Peoples R China
Dai, Taiqiang
[1
]
Zong, Chunlin
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Fourth Mil Med Univ, Sch Stomatol, Dept Oral & Maxillofacial Surg, State Key Lab Mil Stomatol, Xian 710032, Peoples R ChinaFourth Mil Med Univ, Sch Stomatol, Dept Oral & Maxillofacial Surg, State Key Lab Mil Stomatol, Xian 710032, Peoples R China
Zong, Chunlin
[1
]
Liu, Yanpu
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Fourth Mil Med Univ, Sch Stomatol, Dept Oral & Maxillofacial Surg, State Key Lab Mil Stomatol, Xian 710032, Peoples R ChinaFourth Mil Med Univ, Sch Stomatol, Dept Oral & Maxillofacial Surg, State Key Lab Mil Stomatol, Xian 710032, Peoples R China
Liu, Yanpu
[1
]
Liu, Bin
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Fourth Mil Med Univ, Sch Stomatol, Dept Oral Biol, State Key Lab Mil Stomatol, Xian 710032, Peoples R ChinaFourth Mil Med Univ, Sch Stomatol, Dept Oral & Maxillofacial Surg, State Key Lab Mil Stomatol, Xian 710032, Peoples R China
Liu, Bin
[2
]
机构:
[1] Fourth Mil Med Univ, Sch Stomatol, Dept Oral & Maxillofacial Surg, State Key Lab Mil Stomatol, Xian 710032, Peoples R China
[2] Fourth Mil Med Univ, Sch Stomatol, Dept Oral Biol, State Key Lab Mil Stomatol, Xian 710032, Peoples R China
To determine the effect of adipose-derived stem cells (ADSCs) added to bone marrow-derived mesenchymal stem cell (MSC) sheets on bone formation at an ectopic site. We isolated MSCs and ADSCs from the same rabbits. We then prepared MSC sheets for implantation with or without ADSCs subcutaneously in the backs of severe combined immunodeficiency (SCID) mice. We assessed bone formation at eight weeks after implantation by micro-computed tomography and histological analysis. In osteogenic medium, MSCs grew to form multilayer sheets containing many calcium nodules. MSC sheets without ADSCs formed bone-like tissue; although neo-bone and cartilage-like tissues were sparse and unevenly distributed by eight weeks after implantation. In comparison, MSC sheets with ADSCs promoted better bone regeneration as evidenced by the greater density of bone, increased mineral deposition, obvious formation of blood vessels, large number of interconnected ossified trabeculae and woven bone structures, and greater bone volume/total volume within the composite constructs. Our results indicate that although sheets of only MSCs have the potential to form tissue engineered bone at an ectopic site, the addition of ADSCs can significantly increase the osteogenic potential of MSC sheets. Thus, the combination of MSC sheets with ADSCs may be regarded as a promising therapeutic strategy to stimulate bone regeneration.
机构:
Univ Calif San Francisco, Dept Urol, Sch Med, Knuppe Mol Urol Lab, San Francisco, CA 94143 USAUniv Calif San Francisco, Dept Urol, Sch Med, Knuppe Mol Urol Lab, San Francisco, CA 94143 USA
Lin, G.
Qiu, X.
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Univ Calif San Francisco, Dept Urol, Sch Med, Knuppe Mol Urol Lab, San Francisco, CA 94143 USA
Nanjing Univ, Sch Med, Affiliated Drum Tower Hosp, Dept Urol, Nanjing, Jiangsu, Peoples R ChinaUniv Calif San Francisco, Dept Urol, Sch Med, Knuppe Mol Urol Lab, San Francisco, CA 94143 USA
Qiu, X.
Fandel, T.
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Univ Calif San Francisco, Dept Urol, Sch Med, Knuppe Mol Urol Lab, San Francisco, CA 94143 USAUniv Calif San Francisco, Dept Urol, Sch Med, Knuppe Mol Urol Lab, San Francisco, CA 94143 USA
Fandel, T.
Banie, L.
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Univ Calif San Francisco, Dept Urol, Sch Med, Knuppe Mol Urol Lab, San Francisco, CA 94143 USAUniv Calif San Francisco, Dept Urol, Sch Med, Knuppe Mol Urol Lab, San Francisco, CA 94143 USA
Banie, L.
Wang, G.
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Univ Calif San Francisco, Dept Urol, Sch Med, Knuppe Mol Urol Lab, San Francisco, CA 94143 USAUniv Calif San Francisco, Dept Urol, Sch Med, Knuppe Mol Urol Lab, San Francisco, CA 94143 USA
Wang, G.
Lue, T. F.
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Univ Calif San Francisco, Dept Urol, Sch Med, Knuppe Mol Urol Lab, San Francisco, CA 94143 USAUniv Calif San Francisco, Dept Urol, Sch Med, Knuppe Mol Urol Lab, San Francisco, CA 94143 USA
Lue, T. F.
Lin, C-S
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Univ Calif San Francisco, Dept Urol, Sch Med, Knuppe Mol Urol Lab, San Francisco, CA 94143 USAUniv Calif San Francisco, Dept Urol, Sch Med, Knuppe Mol Urol Lab, San Francisco, CA 94143 USA
机构:
Capital Med Univ, Xuanwu Hosp, Dept Vasc Surg, Beijing, Peoples R China
Capital Med Univ, Inst Vasc Surg, Beijing, Peoples R China
Yale Sch Med, Dept Surg, New Haven, CT USA
Yale Sch Med, Vasc Biol & Therapeut Program, New Haven, CT USACapital Med Univ, Xuanwu Hosp, Dept Vasc Surg, Beijing, Peoples R China
Guo, Jianming
Hu, Haidi
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Yale Sch Med, Dept Surg, New Haven, CT USA
China Med Univ, Hosp 1, Dept Surg, Shenyang, Liaoning, Peoples R China
Yale Sch Med, Vasc Biol & Therapeut Program, New Haven, CT USACapital Med Univ, Xuanwu Hosp, Dept Vasc Surg, Beijing, Peoples R China
Hu, Haidi
Gorecka, Jolanta
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Yale Sch Med, Dept Surg, New Haven, CT USA
Yale Sch Med, Vasc Biol & Therapeut Program, New Haven, CT USACapital Med Univ, Xuanwu Hosp, Dept Vasc Surg, Beijing, Peoples R China
Gorecka, Jolanta
Bai, Hualong
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Yale Sch Med, Dept Surg, New Haven, CT USA
Yale Sch Med, Vasc Biol & Therapeut Program, New Haven, CT USACapital Med Univ, Xuanwu Hosp, Dept Vasc Surg, Beijing, Peoples R China
Bai, Hualong
He, Hao
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Yale Sch Med, Dept Surg, New Haven, CT USA
Yale Sch Med, Vasc Biol & Therapeut Program, New Haven, CT USACapital Med Univ, Xuanwu Hosp, Dept Vasc Surg, Beijing, Peoples R China
He, Hao
Assi, Roland
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Yale Sch Med, Dept Surg, New Haven, CT USA
Yale Sch Med, Vasc Biol & Therapeut Program, New Haven, CT USACapital Med Univ, Xuanwu Hosp, Dept Vasc Surg, Beijing, Peoples R China
Assi, Roland
Isaji, Toshihiko
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Yale Sch Med, Dept Surg, New Haven, CT USA
Yale Sch Med, Vasc Biol & Therapeut Program, New Haven, CT USACapital Med Univ, Xuanwu Hosp, Dept Vasc Surg, Beijing, Peoples R China
Isaji, Toshihiko
Wang, Tun
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Yale Sch Med, Dept Surg, New Haven, CT USA
Yale Sch Med, Vasc Biol & Therapeut Program, New Haven, CT USACapital Med Univ, Xuanwu Hosp, Dept Vasc Surg, Beijing, Peoples R China
Wang, Tun
Setia, Ocean
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Yale Sch Med, Dept Surg, New Haven, CT USA
Yale Sch Med, Vasc Biol & Therapeut Program, New Haven, CT USACapital Med Univ, Xuanwu Hosp, Dept Vasc Surg, Beijing, Peoples R China
Setia, Ocean
Lopes, Lara
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Yale Sch Med, Dept Surg, New Haven, CT USA
Yale Sch Med, Vasc Biol & Therapeut Program, New Haven, CT USACapital Med Univ, Xuanwu Hosp, Dept Vasc Surg, Beijing, Peoples R China
Lopes, Lara
Gu, Yongquan
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Capital Med Univ, Xuanwu Hosp, Dept Vasc Surg, Beijing, Peoples R China
Capital Med Univ, Inst Vasc Surg, Beijing, Peoples R ChinaCapital Med Univ, Xuanwu Hosp, Dept Vasc Surg, Beijing, Peoples R China
Gu, Yongquan
Dardik, Alan
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Yale Sch Med, Dept Surg, New Haven, CT USA
VA Connecticut Healthcare Syst, Dept Surg, West Haven, CT USA
Yale Sch Med, Vasc Biol & Therapeut Program, New Haven, CT USACapital Med Univ, Xuanwu Hosp, Dept Vasc Surg, Beijing, Peoples R China
Dardik, Alan
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY,
2018,
315
(06):
: C885
-
C896
机构:
NYU, Langone Med Ctr, Inst Reconstruct Plast Surg Labs, New York, NY 10016 USANYU, Langone Med Ctr, Inst Reconstruct Plast Surg Labs, New York, NY 10016 USA
Valenzuela, Cristian D.
Allori, Alexander C.
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NYU, Langone Med Ctr, Inst Reconstruct Plast Surg Labs, New York, NY 10016 USANYU, Langone Med Ctr, Inst Reconstruct Plast Surg Labs, New York, NY 10016 USA
Allori, Alexander C.
Reformat, Derek D.
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NYU, Langone Med Ctr, Inst Reconstruct Plast Surg Labs, New York, NY 10016 USANYU, Langone Med Ctr, Inst Reconstruct Plast Surg Labs, New York, NY 10016 USA
Reformat, Derek D.
Sailon, Alexander M.
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NYU, Langone Med Ctr, Inst Reconstruct Plast Surg Labs, New York, NY 10016 USANYU, Langone Med Ctr, Inst Reconstruct Plast Surg Labs, New York, NY 10016 USA
Sailon, Alexander M.
Allen, Robert J., Jr.
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NYU, Langone Med Ctr, Inst Reconstruct Plast Surg Labs, New York, NY 10016 USANYU, Langone Med Ctr, Inst Reconstruct Plast Surg Labs, New York, NY 10016 USA
Allen, Robert J., Jr.
Davidson, Edward H.
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NYU, Langone Med Ctr, Inst Reconstruct Plast Surg Labs, New York, NY 10016 USANYU, Langone Med Ctr, Inst Reconstruct Plast Surg Labs, New York, NY 10016 USA
Davidson, Edward H.
Alikhani, Mani
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NYU, Coll Dent, Dept Orthodont, New York, NY 10016 USANYU, Langone Med Ctr, Inst Reconstruct Plast Surg Labs, New York, NY 10016 USA
Alikhani, Mani
Bromage, Timothy G.
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NYU, Coll Dent, Dept Biomat & Biomimet, New York, NY 10016 USANYU, Langone Med Ctr, Inst Reconstruct Plast Surg Labs, New York, NY 10016 USA
Bromage, Timothy G.
Ricci, John L.
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NYU, Coll Dent, Dept Biomat & Biomimet, New York, NY 10016 USANYU, Langone Med Ctr, Inst Reconstruct Plast Surg Labs, New York, NY 10016 USA
Ricci, John L.
Warren, Stephen M.
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NYU, Langone Med Ctr, Inst Reconstruct Plast Surg Labs, New York, NY 10016 USANYU, Langone Med Ctr, Inst Reconstruct Plast Surg Labs, New York, NY 10016 USA
机构:
Third Mil Med Univ, Daping Hosp, Dept Anesthesiol, Chongqing 400042, Peoples R ChinaThird Mil Med Univ, Inst Surg Res, Dept 4, Chongqing 400042, Peoples R China
Chen, Qiang
Yang, Zailiang
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Third Mil Med Univ, Inst Surg Res, Dept 4, Chongqing 400042, Peoples R ChinaThird Mil Med Univ, Inst Surg Res, Dept 4, Chongqing 400042, Peoples R China
Yang, Zailiang
Sun, Shijin
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Third Mil Med Univ, Daping Hosp, Inst Surg Res, Ctr Trauma, Chongqing 400042, Peoples R ChinaThird Mil Med Univ, Inst Surg Res, Dept 4, Chongqing 400042, Peoples R China
Sun, Shijin
Huang, Hong
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Third Mil Med Univ, Daping Hosp, Inst Surg Res, Ctr Trauma, Chongqing 400042, Peoples R ChinaThird Mil Med Univ, Inst Surg Res, Dept 4, Chongqing 400042, Peoples R China
Huang, Hong
Sun, Xinjun
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89th Hosp PLA, Weifang, Peoples R ChinaThird Mil Med Univ, Inst Surg Res, Dept 4, Chongqing 400042, Peoples R China
Sun, Xinjun
Wang, Zhengguo
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Third Mil Med Univ, Inst Surg Res, Dept 4, Chongqing 400042, Peoples R ChinaThird Mil Med Univ, Inst Surg Res, Dept 4, Chongqing 400042, Peoples R China
Wang, Zhengguo
Zhang, Yong
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Third Mil Med Univ, Southwest Hosp, Dept Hematol, Chongqing 400042, Peoples R ChinaThird Mil Med Univ, Inst Surg Res, Dept 4, Chongqing 400042, Peoples R China
Zhang, Yong
Zhang, Bo
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Third Mil Med Univ, Inst Surg Res, Dept 4, Chongqing 400042, Peoples R ChinaThird Mil Med Univ, Inst Surg Res, Dept 4, Chongqing 400042, Peoples R China