Simultaneous determination of nine lignans using pressurized liquid extraction and HPLC-DAD in the fruits of Schisandra chinensis

被引：60

作者：

Lee, Hee Ju ^{[1
]}

Kim, Chul Young ^{[1
]}

机构：

[1] KIST Gangneung Inst, Nat Prod Res Ctr, Kangnung 210340, Gangwon Do, South Korea

来源：

FOOD CHEMISTRY | 2010年 / 120卷 / 04期

关键词：

Schisandra chinensis; Lignans; Pressurized liquid extraction; High performance liquid chromatography-photodiode array detector; COUNTER-CURRENT CHROMATOGRAPHY; SCHIZANDRA-CHINENSIS; GOMISIN-A; CONSTITUENTS; BAILL; SEPARATION;

D O I：

10.1016/j.foodchem.2009.11.068

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The pressurized liquid extraction and HPLC-DAD was developed for extraction and determination of bioactive lignans in Schisandra chinensis. The efficient PLE conditions employed methanol as extraction solvent, 125 degrees C of extraction temperature, 5 min of static extraction time and only one cycle. A rapid HPLC-DAD method was described for simultaneous determination of nine lignans, including schisandrol A, gomisin J, schisandrol B, tigloylgomisin H, angeloylgomisin H, schisandrin A, gamma-schisandrin, gomisin N and schisandrin C. The extraction efficiency of PLE was observed to be comparable with reflux and sonication. In addition, the contents of nine lignans in S. chinensis from different regions were analysed by PLE and HPLC-DAD method. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：1224 / 1228

页数：5

共 21 条

[1]

ATTAWADEE SY, 2006, J CHROMATOGR A, V1125, P172

[2] Pressurised liquid extraction of persistent organic pollutants in environmental analysis [J].

Björklund, E ;

Nilsson, T ;

Bowadt, S .

TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2000, 19 (07) :434-445

[3] Optimum SFE condition for lignans of Schisandra chinensis fruits [J].

Choi, YH ;

Kim, J ;

Jeon, SH ;

Yoo, KP ;

Lee, HK .

CHROMATOGRAPHIA, 1998, 48 (9-10) :695-699

[4] Validation of a method for the simultaneous determination of four schisandra lignans in the raw herb and commercial dried aqueous extracts of Schisandra chinensis (Wu Wei Zi) by RP-LC with DAD [J].

Halstead, C. W. ;

Lee, S. ;

Khoo, C. S. ;

Hennell, J. R. ;

Bensoussan, A. .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2007, 45 (01) :30-37

[5] VALIDITY OF THE ORIENTAL MEDICINES .60. ANTIHEPATOTOXIC ACTIONS OF LIGNOIDS FROM SCHIZANDRA-CHINENSIS FRUITS [J].

HIKINO, H ;

KISO, Y ;

TAGUCHI, H ;

IKEYA, Y .

PLANTA MEDICA, 1984, 50 (03) :213-218

[6] Preparative separation and purification of deoxyschisandrin and γ-schisandrin from Schisandra chinensis (Turcz.) Baill by high-speed counter-current chromatography [J].

Huang, TH ;

Shen, PN ;

Shen, YJ .

JOURNAL OF CHROMATOGRAPHY A, 2005, 1066 (1-2) :239-242

[7] Acetylcholinesterase inhibitory effect of lignans isolated from Schizandra chinensis [J].

Hung, Tran Manh ;

Na, MinKyun ;

Min, Byung Sun ;

Ngoc, Tran Minh ;

Lee, IkSoo ;

Zhang, XinFeng ;

Bae, KiHwan .

ARCHIVES OF PHARMACAL RESEARCH, 2007, 30 (06) :685-690

[8]

IKEYA Y, 1979, CHEM PHARM BULL, V27, P1576

[9]

IKEYA Y, 1982, CHEM PHARM BULL, V30, P132

[10] THE CONSTITUENTS OF SCHIZANDRA CHINENSIS .14. A LIGNAN FROM SCHIZANDRA CHINENSIS [J].

IKEYA, Y ;

TAGUCHI, H ;

MITSUHASHI, H ;

TAKEDA, S ;

KASE, Y ;

ABURADA, M .

PHYTOCHEMISTRY, 1988, 27 (02) :569-573

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