Interaction of hTCF4 by yeast two-hybrid system

被引：1
作者：
Lin, GT [1 ]
Zhang, ZW
Zang, T
Xin, DQ
Chang, ZJ
Guo, YL
机构：
[1] Beijing Med Univ, Inst Urol, Beijing 100034, Peoples R China
[2] Beijing Med Univ, Dept Physiol, Beijing 100034, Peoples R China
[3] Qinghua Univ, Dept Biol, Beijing 100084, Peoples R China
来源：
CHINESE SCIENCE BULLETIN | 2000年 / 45卷 / 21期
基金：
中国国家自然科学基金;
关键词：
Wnt/frizzled; hTCF4; yeast two-hybrid;
D O I：
10.1007/BF02909690
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
To investigate the interaction of hTCF4, the yeast two-hybrid system has been used for testing the interaction of mutants of hTCF4 with themselves. Mutants of hTCF4(hTCF4 I) and hTCF4 II) have been obtained by polymerase chain reaction (PCR). Bait (hTCF4 I-pDBLeu and hTCF4 II-pDBLeu) and prey (hTCF4 I-pPC86 and hTCF4 II-pPC86) have been constructed by DNA recombination for yeast two-hybrid. Interaction of hTCF4 II with itself is found in the reverse yeast two-hybrid system (GIBCOBRL Co.). However, no interactions are found in hTCF4 II with hTCF4 I and in hTCF4 I with hTCF4 I. These results suggest that hTCF4 could interact with itself to form homodimer or homocopolymer and perform the transcriptional activating function through LZ or HLH motifs in nucleus of renal cell carcinoma.
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收藏
页码：1973 / 1977
页数：5
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