A novel mitochondria-targeted phosphorescence probe for hypochlorite ions detection in living cells

被引：16

作者：

Yi, Sili ^{[1
,3
]}

Lu, Zhen ^{[1
]}

Lin, Yandai ^{[1
]}

Wang, Jun ^{[2
]}

Qiao, Ziwen ^{[1
]}

Shen, Rongkai ^{[4
]}

Zhang, Jin ^{[1
]}

Hou, Linxi ^{[1
]}

机构：

[1] Fuzhou Univ, Coll Chem Engn, Fuzhou 350116, Peoples R China

[2] Fuzhou Univ, Coll Chem, Fuzhou 350116, Peoples R China

[3] Fuzhou Univ, Coll Chem, Inst Food Safety & Environm Monitoring Photocatal, Fuzhou 350116, Peoples R China

[4] Fujian Med Univ, Affiliated Hosp 1, Dept Orthopaed Surg, Fuzhou 350116, Peoples R China

来源：

TALANTA | 2020年 / 209卷

基金：

中国国家自然科学基金;

关键词：

Gemini-iridium complex; Isoniazide; Mitochondria-targeted; Hypochlorite anion; Chemo-sensor; FLUORESCENT-PROBE; IRIDIUM(III) COMPLEXES; RATIOMETRIC DETECTION; SELECTIVE DETECTION; ACID DETECTION; FAST-RESPONSE;

D O I：

10.1016/j.talanta.2019.120516

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Monitoring hypochlorite anion (ClO-) in living cells is particularly meaningful and valuable, because overexposure of the ClO- may cause a potential health hazard towards animals and humans. Considering the special structure and properties of the gemini surfactant, a novel amphiphilic gemini-iridium complex Ir[(ppy-iso)(2)(bpy-tma(2)Br(2))] (Ir-iso) with isoniazide as a recognition site for ClO- was designed. The Ir-iso possessed an excellent water-solubility as well as a strong ClO- binding capacity, as revealed from the rapid response of emission signal towards ClO-. It was worth noting that such probe had a highly-specific selectivity with a low detection limit (20.5 nM) and was suitable in physiological environment. The cell viability assay, cell imaging, and co-location studies further proved that the Ir-iso had little cytotoxicity and was specifically localized in the mitochondria of breast cancer cells, being a promising candidate of chemo-sensor to detect the endogenous ClO- in living cells.

引用

页数：7

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