Fast volumetric imaging with patterned illumination via digital micro-mirror device-based temporal focusing multiphoton microscopy

被引:23
作者
Chang, Chia-Yuan [1 ,2 ]
Hu, Yvonne Yuling [3 ]
Lin, Chun-Yu [1 ,2 ]
Lin, Cheng-Han [1 ]
Chang, Hsin-Yu [1 ,2 ]
Tsai, Sheng-Feng [4 ]
Lin, Tzu-Wei [5 ]
Chen, Shean-Jen [1 ,2 ,6 ]
机构
[1] Natl Cheng Kung Univ, Dept Engn Sci, Tainan 701, Taiwan
[2] Natl Cheng Kung Univ, Ctr Micro Nano Sci & Technol, Tainan 701, Taiwan
[3] Natl Cheng Kung Univ, Dept Photon, Tainan 701, Taiwan
[4] Natl Cheng Kung Univ, Inst Basic Med Sci, Tainan 701, Taiwan
[5] Univ Tsukuba, Fac Hlth & Sport Sci, Tsukuba, Ibaraki 3058574, Japan
[6] Natl Cheng Kung Univ, Adv Optoelect Technol Ctr, Tainan 701, Taiwan
关键词
EXCITATION MICROSCOPY; FLUORESCENCE; RESOLUTION; DYNAMICS; TRACKING; TISSUE;
D O I
10.1364/BOE.7.001727
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Temporal focusing multiphoton microscopy (TFMPM) has the advantage of area excitation in an axial confinement of only a few microns; hence, it can offer fast three-dimensional (3D) multiphoton imaging. Herein, fast volumetric imaging via a developed digital micromirror device (DMD)-based TFMPM has been realized through the synchronization of an electron multiplying charge-coupled device (EMCCD) with a dynamic piezoelectric stage for axial scanning. The volumetric imaging rate can achieve 30 volumes per second according to the EMCCD frame rate of more than 400 frames per second, which allows for the 3D Brownian motion of one-micron fluorescent beads to be spatially observed. Furthermore, it is demonstrated that the dynamic HiLo structural multiphoton microscope can reject background noise by way of the fast volumetric imaging with high-speed DMD patterned illumination. (C) 2016 Optical Society of America
引用
收藏
页码:1727 / 1736
页数:10
相关论文
共 38 条
[1]   Multifocal multiphoton microscopy [J].
Bewersdorf, J ;
Pick, R ;
Hell, SW .
OPTICS LETTERS, 1998, 23 (09) :655-657
[2]   Simultaneous two-photon calcium imaging at different depths with spatiotemporal multiplexing [J].
Cheng, Adrian ;
Goncalves, J. Tiago ;
Golshani, Peyman ;
Arisaka, Katsushi ;
Portera-Cailliau, Carlos .
NATURE METHODS, 2011, 8 (02) :139-U58
[3]   Nonlinear structured-illumination enhanced temporal focusing multiphoton excitation microscopy with a digital micromirror device [J].
Cheng, Li-Chung ;
Lien, Chi-Hsiang ;
Da Sie, Yong ;
Hu, Yvonne Yuling ;
Lin, Chun-Yu ;
Chien, Fan-Ching ;
Xu, Chris ;
Dong, Chen Yuan ;
Chen, Shean-Jen .
BIOMEDICAL OPTICS EXPRESS, 2014, 5 (08) :2526-2536
[4]   Spatiotemporal focusing-based widefield multiphoton microscopy for fast optical sectioning [J].
Cheng, Li-Chung ;
Chang, Chia-Yuan ;
Lin, Chun-Yu ;
Cho, Keng-Chi ;
Yen, Wei-Chung ;
Chang, Nan-Shan ;
Xu, Chris ;
Dong, Chen Yuan ;
Chen, Shean-Jen .
OPTICS EXPRESS, 2012, 20 (08) :8939-8948
[5]   Improvement of axial resolution and contrast in temporally focused widefield two-photon microscopy with structured light illumination [J].
Choi, Heejin ;
Yew, Elijah Y. S. ;
Hallacoglu, Bertan ;
Fantini, Sergio ;
Sheppard, Colin J. R. ;
So, Peter T. C. .
BIOMEDICAL OPTICS EXPRESS, 2013, 4 (07) :995-1005
[6]   3D-resolved fluorescence and phosphorescence lifetime imaging using temporal focusing wide-field two-photon excitation [J].
Choi, Heejin ;
Tzeranis, Dimitrios S. ;
Cha, Jae Won ;
Clemenceau, Philippe ;
de Jong, Sander J. G. ;
van Geest, Lambertus K. ;
Moon, Joong Ho ;
Yannas, Ioannis V. ;
So, Peter T. C. .
OPTICS EXPRESS, 2012, 20 (24) :26219-26235
[7]   Remotely scanned multiphoton temporal focusing by axial grism scanning [J].
Dana, Hod ;
Shoham, Shy .
OPTICS LETTERS, 2012, 37 (14) :2913-2915
[8]   Simultaneous multiplane confocal microscopy using acoustic tunable lenses [J].
Duocastella, Marti ;
Vicidomini, Giuseppe ;
Diaspro, Alberto .
OPTICS EXPRESS, 2014, 22 (16) :19293-19301
[9]   Breakthroughs in Photonics 2014: Spatiotemporal Focusing: Advances and Applications [J].
Durfee, C. G. ;
Squier, J. A. .
IEEE PHOTONICS JOURNAL, 2015, 7 (03)
[10]   Rapid 3D light-sheet microscopy with a tunable lens [J].
Fahrbach, Florian O. ;
Voigt, Fabian F. ;
Schmid, Benjamin ;
Helmchen, Fritjof ;
Huisken, Jan .
OPTICS EXPRESS, 2013, 21 (18) :21010-21026