Determination of dexamethasone acetate in cream by HPLC

被引：36

作者：

Garcia, CV ^{[1
]}

Breier, AR ^{[1
]}

Steppe, M ^{[1
]}

Schapoval, EES ^{[1
]}

Oppe, TP ^{[1
]}

机构：

[1] Univ Fed Rio Grande Sul, Fac Farm, Dept Prod & Control Medicamentos, BR-90610000 Porto Alegre, RS, Brazil

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2003年 / 31卷 / 03期

关键词：

dexamethasone; validation of method; HPLC; quality control;

D O I：

10.1016/S0731-7085(02)00695-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The aim of this research was to validate a high performance liquid chromatographic method for the quantitative determination of dexamethasone acetate contained in cream preparation. A MetaSil octadecyl silane (250 x 4.6 mm, 5 mum) column, a methanol: water (65:35; v/v) mobile phase (1.0 ml min(-1)) and an UV detector (set at 254 nm) were used to evaluate the parameters: linearity, precision, accuracy, specificity, as well as, quantitation and detection limits. The calibration curve showed a correlation coefficient of 0.9999. The precision was demonstrated by the relative standard deviation (RSD) of 0.53. The recovery test resulted in an average of 97.85%, what confirmed the accuracy of the method. The quantitation and detection limits determined were 1.41 and 0.47 mug ml(-1) respectively. The specificity test showed there was no interference in the drug peak. (C) 2003 Elsevier Science B.V. All rights reserved.

引用

页码：597 / 600

页数：4

共 10 条

[1]

[Anonymous], 1996, JAP PHARM

[2]

[Anonymous], 1996, INT C HARM TECHN REQ

[3] ANALYSIS OF DEXAMETHASONE ACETATE IN PHARMACEUTICAL FORMULATIONS BY HPLC [J].