Use of Rotofor preparative isoelectrofocusing cell in protein purification procedure

被引：28
作者：
Ayala, A [1 ]
Parrado, J [1 ]
Machado, A [1 ]
机构：
[1] Univ Sevilla, Fac Farm, Dept Bioquim Bromatol & Toxicol, E-41012 Seville, Spain
来源：
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1998年 / 69卷 / 01期
关键词：
Rotofor; superoxide dismutase; 6-phosphogluconate dehydrogenase;
D O I：
10.1007/BF02786017
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The Rotofor cell is a preparative isoelectric focusing (IEF) apparatus, in which IEF is performed entirely in free solution. Electrofocusing in Rotofor cell has been described as well-suited for use at any stage of a purification scheme. However, it has some important limitations in resolving complex mixtures of proteins. This paper describes the advantages and disadvantages of using the Rotofor cell in purification protocols.
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页码：11 / 16
页数：6
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