A localized chemical pulse generator for the development of a microfluidic cell-based biosensorPeng

被引:10
作者
Chen, Peng [1 ]
Guo, Yiran [1 ]
Wang, Jie [1 ]
Du, Wei [1 ]
Feng, Xiaojun [1 ]
Liu, Bi-Feng [1 ]
机构
[1] Huazhong Univ Sci & Technol, Key Lab Biomed Photon MOE, Wuhan Natl Lab Optoelect,Dept Biomed Engn,Coll Li, Hubei Bioinformat & Mol Imaging Key Lab,Syst Biol, Wuhan 430074, Hubei, Peoples R China
基金
中国国家自然科学基金; 国家重点研发计划;
关键词
based biosensor; Microfluidic chip; Localized chemical pulse; Calcium imaginga; ENZYME-BASED BIOSENSORS; RECEPTORS;
D O I
10.1016/j.snb.2017.05.038
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Cell-based biosensor (CBB) has shown potential to provide a rapid and sensitive measurement means for monitoring specific biological substances. However, the detecting frequency and reusability remain a challenge for further application of CBB. In this paper, a microfluidic cell-based biosensor (mu CBB) was developed, which consisted of a localized millisecond chemical pulse as sample introduction for pulsing individual cells without interfering with adjacent cells and single HeLa cells expressing endogenous P2Y receptors as the sensing elements. Gated injection of pinched flow was used to generate millisecond ATP pulses with predefined width. By integrating localized ATP pulse with predefined width, multiple sensing elements could be used to detect ATP pulses without impact on their functionality. Thus, the detecting frequency of the developed mu CBB is multiplied by the number of available cells in comparison to that reported previously. We expect the developed method to be potentially useful to enhance the usability of CBB for biological and biomedical analysis. (C) 2017 Elsevier B.V. All rights reserved.
引用
收藏
页码:112 / 119
页数:8
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