Development of a fluorescence polarization immunoassay for polycyclic aromatic hydrocarbons

被引：17
作者：
Goryacheva, Irina Yu
Eremin, Sergei A. [1 ]
Shutaleva, Ekaterina A.
Suchanek, Miloslav
Niessner, Reinhard
Knopp, Dietmar
机构：
[1] Moscow MV Lomonosov State Univ, Dept Chem Enzymol, Moscow 119992, Russia
[2] EXBIO Praha As, Vestec U Prahy, Czech Republic
[3] Tech Univ Munich, Inst Hydrochem & Chem Balneol, D-8000 Munich, Germany
来源：
ANALYTICAL LETTERS | 2007年 / 40卷 / 07期
关键词：
fluorescence polarization immunoassay; PAHs; screening;
D O I：
10.1080/00032710701297034
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A fluorescence polarization immunoassay for detection of polycyclic aromatic hydrocarbons (PAHs) was developed. Fluorescein-labeled tracers based on different PAHs were synthesized and studied in order to achieve a high sensitivity using both monoclonal and polyclonal antibodies. The application of different combinations of antibody tracer pairs allows to separately determine groups of small and large PAHs or to realize a class-specific assay. The limits of detection of PAHs were 0.9, 1.1, and 3.4 ng ml 21 for benzo[a] pyrene, naphthalene, and anthracene, respectively. The developed fluorescence polarization immunoassay could be used for rapid screening of natural water samples for contamination with PAHs.
引用
收藏
页码：1445 / 1460
页数：16
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