Identification and characterization of a bacterial chromosome partitioning site

被引：351

作者：

Lin, DCH ^{[1
]}

Grossman, AD ^{[1
]}

机构：

[1] MIT, Dept Biol, Cambridge, MA 02139 USA

来源：

CELL | 1998年 / 92卷 / 05期

关键词：

D O I：

10.1016/S0092-8674(00)81135-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have identified a DNA site involved in chromosome partitioning in B. subtilis. This site was identified in vivo as the binding site for the chromosome partitioning protein Spo0J, a member of the ParB family of partitioning proteins. Spo0J is a site-specific DNA-binding protein that recognizes a 16 bp sequence found in spo0J. Allowing two mismatches, this sequence occurs ten times in the entire B. subtilis chromosome, all in the origin-proximal similar to 20%. Eight of the ten sequences are bound to Spo0J in vivo. The presence of a site on an otherwise unstable plasmid stabilized the plasmid in a Spo0J-dependent manner, demonstrating that this site, called parS, can function as a partitioning site. This site and Spo0J are conserved in a wide range of bacterial species.

引用

收藏

页码：675 / 685

页数：11

相关论文

共 33 条

[1] PARTITION-MEDIATED INCOMPATIBILITY OF BACTERIAL PLASMIDS [J].

AUSTIN, S ;

NORDSTROM, K .

CELL, 1990, 60 (03) :351-354

[2]

AUSUBEL FM, 1990, CURRENT PROTOCOLS MO

[3] Holding chromatids together to ensure they go their separate ways [J].

Bickel, SE ;

OrrWeaver, TL .

BIOESSAYS, 1996, 18 (04) :293-300

[4] Dynamic, mitotic-like behavior of a bacterial protein required for accurate chromosome partitioning [J].

Glaser, P ;

Sharpe, ME ;

Raether, B ;

Perego, M ;

Ohlsen, K ;

Errington, J .

GENES & DEVELOPMENT, 1997, 11 (09) :1160-1168

[5] Chromosome and low copy plasmid segregation in E-coli: Visual evidence for distinct mechanisms [J].

Gordon, GS ;

Sitnikov, D ;

Webb, CD ;

Teleman, A ;

Straight, A ;

Losick, R ;

Murray, AW ;

Wright, A .

CELL, 1997, 90 (06) :1113-1121

[6] Genetic networks controlling the initiation of sporulation and the development of genetic competence in Bacillus subtilis [J].

Grossman, AD .

ANNUAL REVIEW OF GENETICS, 1995, 29 :477-508

[7] A direct link between sister chromatid cohesion and chromosome condensation revealed through the analysis of MCD1 in S-cerevisiae [J].

Guacci, V ;

Koshland, D ;

Strunnikov, A .

CELL, 1997, 91 (01) :47-57

[8]

Harwood C. R., 1990, MOL BIOL METHODS BAC

[9] Suppression of initiation defects of chromosome replication in Bacillus subtilis dnaA and oriC-deleted mutants by integration of a plasmid replicon into the chromosomes [J].

Hassan, AKM ;

Moriya, S ;

Ogura, M ;

Tanaka, T ;

Kawamura, F ;

Ogasawara, N .

JOURNAL OF BACTERIOLOGY, 1997, 179 (08) :2494-2502

[10] Spreading of transcriptional repressor SIR3 from telomeric heterochromatin [J].

Hecht, A ;

StrahlBolsinger, S ;

Grunstein, M .

NATURE, 1996, 383 (6595) :92-96

← 1 2 3 4 →