Investigation of Extracellular Vesicles From SARS-CoV-2 Infected Specimens: A Safety Perspective

被引：9
作者：
Nunez Lopez, Yury O. [1 ]
Casu, Anna [1 ]
Pratley, Richard E. [1 ]
机构：
[1] AdventHealth, Translat Res Inst, Orlando, FL 32803 USA
来源：
FRONTIERS IN IMMUNOLOGY | 2021年 / 12卷
关键词：
extracellular vesicle; Trojan exosome; Trojan EV; SARS-CoV-2; COVID-19; laboratory safety; PROMOTES;
D O I：
10.3389/fimmu.2021.617042
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
The coronavirus disease 2019 (COVID-19) pandemic, caused by the SARS-CoV-2 virus, is wreaking havoc around the world. Considering that extracellular vesicles (EVs) released from SARS-CoV-2 infected cells might play a role in a viremic phase contributing to disease progression and that standard methods for EV isolation have been reported to co-isolate viral particles, we would like to recommend the use of heightened laboratory safety measures during the isolation of EVs derived from SARS-CoV-2 infected tissue and blood from COVID-19 patients. Research needs to be conducted to better understand the role of EVs in SARS-CoV-2 infectivity, disease progression, and transmission. EV isolation procedures should include approaches for protection from SARS-CoV-2 contamination. We recommend the EV and virology scientific communities develop collaborative projects where relationships between endogenous EVs and potentially lethal enveloped viruses are addressed to better understand the risks and pathobiology involved.
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页数：8
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