Antimicrobial resistance, penicillin-binding protein sequences, and pilus islet carriage in relation to clonal evolution of Streptococcus pneumoniae serotype 19A in Russia, 2002-2013

被引:19
作者
Mayanskiy, N. [1 ]
Savinova, T. [1 ]
Alyabieva, N. [1 ]
Ponomarenko, O. [1 ]
Brzhozovskaya, E. [1 ]
Lazareva, A. [1 ]
Katosova, L. [1 ]
Kozlov, R. [2 ]
机构
[1] Sci Ctr Childrens Hlth, Lomonosovskiy Ave 2, Moscow 119991, Russia
[2] Smolensk State Med Univ, Inst Antimicrobial Chemotherapy, 46-A Kirova St,POB 5, Smolensk 214019, Russia
关键词
Antimicrobial resistance; multilocus sequence typing; penicillin-binding protein; pneumococcus; serotype; 19A; INVASIVE PNEUMOCOCCAL DISEASE; ACUTE OTITIS-MEDIA; ANTIBIOTIC-RESISTANCE; UNITED-STATES; CHILDREN; VACCINE; PREVALENCE; EXPANSION; MOSCOW; GENES;
D O I
10.1017/S0950268817000541
中图分类号
R1 [预防医学、卫生学];
学科分类号
1004 ; 120402 ;
摘要
Clonal changes of serotype 19A pneumococci have been appreciated in conjunction with growing prevalence of this serotype after implementation of the seven-valent pneumococcal conjugate vaccine (PCV7). In the present study, we characterized serotype 19A pneumococci collected in Russia within a decade preceding the implementation of PCV vaccination and described their clonal evolution. We retrospectively analyzed non-invasive serotype 19A isolates collected in 2002-2013. All isolates were subjected to multilocus sequence typing, antimicrobial susceptibility testing, determination of macrolide resistance genotype, molecular detection of pilus islet (PI) carriage, sequencing of penicillin-binding protein (PBP) genes. A total of 49 serotype 19A isolates represented 25 sequence types, of which 14 were newly described. The majority of isolates were distributed among clonal complex (CC) 663 (28%), CC230 (25%), CC156, and CC320 (14% each). CC663 and CC156 dominated in 2003, but were replaced by CC230 and CC320 later on; CC320 was only evident starting 2010. All isolates of CC663 and CC156 carried PI1; CC320 possessed both PI1 and PI2. The overall rate of altered amino acids in penicillin-nonsusceptible isolates was 13.9%, 7.2%, and 8.7% for PBP1a, PBP2b, and PBP2x, respectively. Our findings demonstrate that the clonal structure of serotype 19A pneumococci may evolve without PCV pressure.
引用
收藏
页码:1708 / 1719
页数:12
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