Determination of methocarbamol concentration in human plasma by high performance liquid chromatography-tandem mass spectrometry

被引：25

作者：

Zha, Wuyi ^{[1
]}

Zhu, Zhimeng ^{[1
]}

机构：

[1] Pharmascience, Montreal, PQ H4P 2T4, Canada

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2010年 / 878卷 / 9-10期

关键词：

Methocarbamol; LC/MS/MS; Bioequivalence; Human plasma; METABOLISM;

D O I：

10.1016/j.jchromb.2010.01.025

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple and reproducible high performance liquid chromatography-tandem mass spectrometric method was developed for methocarbamol analysis in human plasma. Methocarbamol and the internal standard (IS) were extracted by a protein precipitation method. Under isocratic separation condition the chromatographic run time was 3.0 min. The calibration curve was linear over a range of 150-12,000 ng/mL with good intraday assay and interday assay precision (CV% < 10.9%). The method was proven to be sensitive and selective for the analysis of methocarbamol in human plasma for bioequivalence study. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：831 / 835

页数：5

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