Ion exchange expanded bed chromatography for the purification of an extracelular inulinase from Kluyveromyces marxianus

被引：28
作者：
Kalil, SJ
Maugeri-Filho, F
Rodrigues, MI
机构：
[1] Fundacao Univ Rio Grande, Dept Chem, BR-96201900 Rio Grande, RS, Brazil
[2] Univ Estadual Campinas, Dept Food Engn, Campinas, SP, Brazil
来源：
PROCESS BIOCHEMISTRY | 2005年 / 40卷 / 02期
关键词：
expanded bed adsorption; inulinase recovery; ion exchange; Kluyveromyces marxianus; streamline SP;
D O I：
10.1016/j.procbio.2004.01.034
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The use of an expanded bed of Streamline SP resin (Pharmacia) for the purification of an extracellular inulinase directly from an unclarified crude broth was investigated. The purification yield achieved 74% with a purification factor of about 10.4. Adsorption were similar whether the crude medium had microbial cells or not. The influence of bed expansion on adsorption performance was determined by frontal analysis. Breakthrough curves for inulinase were compared and the process was more efficient at a bed expansion degree of 2.0 (bed voidage of 0.7). (C) 2004 Elsevier Ltd. All rights reserved.
引用
收藏
页码：581 / 586
页数：6
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