Fluorescence in situ hybridization (FISH): DNA probe production and hybridization criteria

被引：12

作者：

Nath, J ^{[1
]}

Johnson, KL

机构：

[1] W Virginia Univ, Genet & Dev Biol Program, Morgantown, WV 26506 USA

[2] Lawrence Livermore Natl Lab, Biol & Biotechnol Res Program, Livermore, CA 94551 USA

来源：

BIOTECHNIC & HISTOCHEMISTRY | 1998年 / 73卷 / 01期

关键词：

complementary sequences; DNA probes; FISH; fluorescence in situ hybridization; probe production; review;

D O I：

10.3109/10520299809140502

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We describe methods for the production of fluorescence in situ hybridization (FISH) probes and the utilization of these probes for the detection of complementary DNA sequences with accuracy and sensitivity for application in both basic research and clinical diagnosis. Due to the frequent use of FISH in many laboratories, it is important to apply the most convenient and reproducible approach. This review describes some of th most recent techniques, and includes versatile, effective and simple methods of probe production and fluorescence in situ hybridization. We also describe method for the production of region-specific an chromosome-specific DNA probes and hybridization techniques for the visualization of these probes.

引用

页码：6 / 22

页数：17

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