Total serum protein N-glycome profiling on a capillary electrophoresis-microfluidics platform

被引:47
作者
Callewaert, N
Contreras, R
Mitnik-Gankin, L
Carey, L
Matsudaira, P
Ehrlich, D
机构
[1] Whitehead Inst Biomed Res, BioMEMS Lab, Cambridge, MA 02142 USA
[2] State Univ Ghent, Dept Mol Biomed Res, Ghent, Belgium
[3] VIB, Ghent, Belgium
关键词
biomarkers; cross-injector; glycomics; lab-on-a-chip; microfluidics; miniaturization;
D O I
10.1002/elps.200406020
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
We implemented 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled asparagine-linked glycan (N-glycan) profiling on a microfluidic electrophoresis platform. Using 11.5 cm effective length etched channels and 4% linear polyacrylamide as the separation matrix, the major N-glycans in human serum were profiled in 12 min with a resolution comparable to what is achieved for these analytes on gel-based DNA sequencers. This demonstration suggests a practical clinical application for highspeed compact analyzers which might be uniquely based on microfluidic devices.
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页码:3128 / 3131
页数:4
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