Osteoblastic differentiation of human mesenchymal stem cells with platelet lysate

被引：169

作者：

Chevallier, Nathalie ^{[1
,2
]}

Anagnostou, Fani ^{[3
,4
]}

Zilber, Sebastien ^{[1
,5
]}

Bodivit, Gwellaouen ^{[1
,2
]}

Maurin, Sophie ^{[1
,2
]}

Barrault, Aurelie ^{[1
,2
]}

Bierling, Philippe ^{[1
,2
]}

Hernigou, Philippe ^{[1
,5
]}

Layrolle, Pierre ^{[6
]}

Rouard, Helene ^{[1
,2
]}

机构：

[1] Paris EST Univ, Hop Henri Mondor, Cellular & Tissular Bioengn Lab, Paris, France

[2] EFS Ile France, Cell Therapy Facil, F-94010 Creteil, France

[3] Univ Paris 07, CNRS, UFR Odontol, Lab Osteoarticular Engn,UMR 7052, Paris, France

[4] Univ Paris 07, UFR Odontol, Hop La Pitie Salpetriere, AP HP,Dept Periodontol, Paris, France

[5] Hop Henri Mondor, AP HP, Dept Orthopaed Surg, Creteil, France

[6] Univ Nantes, Fac Dent Surg, INSERM, Lab Osteoarticular & Dent Tissue Engn,U791, F-44042 Nantes 1, France

来源：

BIOMATERIALS | 2010年 / 31卷 / 02期

关键词：

Mesenchymal stem cells; Platelet lysate; Osteoblastic gene expression; HA/beta TCP scaffold; MARROW STROMAL CELLS; GROWTH-FACTOR LEVELS; RICH PLASMA; IN-VITRO; OSTEOGENIC DIFFERENTIATION; BONE-FORMATION; EXPANSION; DEXAMETHASONE; PROLIFERATION; EXPRESSION;

D O I：

10.1016/j.biomaterials.2009.09.043

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Culture of expanded mesenchymal stem cells (MSCs) seeded on biomaterials may represent a clinical alternative to autologous bone graft in bone regeneration. Foetal bovine serum (FBS) is currently used for MSC expansion, despite risks of infectious disease transmission and immunological reaction due to its xenogenic origin. This study aimed to compare the osteogenic capacities of clinical-grade human MSCs cultured with FBS or allogenic human platelet lysate (PL). In vitro, MSCs cultured in PL both accelerate the expansion rate over serial passages and spontaneously induce osteoblastic gene expression such as alkaline phosphatase (ALP), bone sialoprotein (BSP), osteopontin (Op) and bone morphogenetic protein-2 (BMP-2). In vivo, ectopic bone formation is only observed on ceramics seeded with MSCs grown in PL medium implanted under the skin of immunodeficient mice for 7 weeks. In conclusion, allogenic human PL accelerates MSC proliferation and enhances MSC osteogenic differentiation. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：270 / 278

页数：9

共 48 条

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