Molecular identification of chlamydial cause of abortion in small ruminants in Jordan

被引：11

作者：

Ababneh, Huthaifa Salah ^{[1
]}

Ababneh, Mustafa Mohammed Kheir ^{[2
]}

Hananeh, Wael Mahmoud ^{[2
]}

Alsheyab, Fawzi Mohammad ^{[1
]}

Jawasreh, Khaleel Ibraheem ^{[3
]}

Al-Gharaibeh, Moath Ahmad ^{[1
]}

Ababneh, Mohammed Mahmoud ^{[2
]}

机构：

[1] Natl Ctr Agr Res & Extens, Baqa 19381, Jordan

[2] Jordan Univ Sci & Technol, Fac Vet Med, Irbid, Jordan

[3] Jordan Univ Sci & Technol, Fac Agr, Irbid, Jordan

来源：

TROPICAL ANIMAL HEALTH AND PRODUCTION | 2014年 / 46卷 / 08期

关键词：

Chlamydophila abortus; Ovine enzootic abortion; Outermembrane protein gene; PCR-RFLP; DNA sequencing; Jordan; CHLAMYDOPHILA-ABORTUS; SHEEP; INFECTIONS; OVINE; DIAGNOSIS; PSITTACI; PECORUM; STRAINS; GOATS;

D O I：

10.1007/s11250-014-0654-x

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Chlamydophila abortus (Ch. abortus) is the etiological agent of ovine enzootic abortion (OEA) and one of the most common infectious agents of abortion in small ruminants worldwide. RFLP-PCR analysis of the outer membrane protein gene (OMP2 gene) was used for diagnosis and characterization of chlamydial causes of abortion in small ruminants in Jordan. Sixty-six placental tissues and 15 vaginal swabs were collected from aborted ewes and does to identify cause of abortion in Jordan. Thirty-eight placental samples (58 %) and 13 vaginal swabs (87 %) were positive for chlamydial DNA. Shedding of bacteria in vaginal swabs was detected within 7 days after abortion. The results of this study showed that chlamydiosis is one of the important causes of abortion in small ruminants in Jordan. In addition, vaginal swab is an excellent sample for molecular diagnosis of chlamydiosis. DNA sequencing and RFLP analysis of the OMP2 reveal that all chlamydial cause of abortion in small ruminants in Jordan are due to Ch. abortus. While, Ch. pecorum was not detected in any sample. OMP2 gene of the isolated Jordanian strain was identical (100 %) to Ch. abortus FAS strain. In conclusion, Ch. abortus is an important cause of abortion in Jordan; vaginal swab within 7 days of abortion can be used for molecular diagnosis of chlamydiosis in small ruminants.

引用

页码：1407 / 1412

页数：6

共 22 条

[1] Ovine and caprine toxoplasmosis (Toxoplasma gondii) in aborted animals in Jordanian goat and sheep flocks [J].