3D-Scaffolds from Poly(3-hydroxybutyrate)-Poly(ethylene glycol) Copolymer for Tissue Engineering

被引：29

作者：

Bonartsev, A. P. ^{[1
,2
,3
]}

Zharkova, I. I. ^{[1
]}

Yakovlev, S. G. ^{[2
,3
]}

Myshkina, V. L. ^{[2
]}

Makhina, T. K. ^{[2
]}

Zernov, A. L. ^{[1
,3
]}

Kudryashova, K. S. ^{[1
]}

Feofanov, A. V. ^{[1
]}

Akulina, E. A. ^{[2
]}

Ivanova, E. V. ^{[1
]}

Zhuikov, V. A. ^{[2
,3
]}

Andreeva, N. V. ^{[1
]}

Voinova, V. V. ^{[1
]}

Bessonov, I. V. ^{[4
]}

Kopitsyna, M. V. ^{[4
]}

Morozov, A. S. ^{[4
]}

Bonartseva, G. A. ^{[2
]}

Shaitan, K. V. ^{[1
]}

Kirpichnikov, M. P. ^{[1
]}

机构：

[1] Moscow MV Lomonosov State Univ, Fac Biol, Leninskie Gory 1-12, Moscow 119236, Russia

[2] Russian Acad Sci, Biotechnol Res Ctr, Alexey Nikolaevich Bach Inst Biochem, 33,Bld 2 Leninsky Ave, Moscow 119071, Russia

[3] Nizhny Novgorod State Med Acad, Minin & Pozharsky Sq 10-1, Nizhnii Novgorod 603950, Russia

[4] Bauman Moscow State Tech Univ, 5,2-Nd Baumanskaya, Moscow 105005, Russia

来源：

JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING | 2016年 / 6卷 / 01期

基金：

俄罗斯科学基金会;

关键词：

Poly(3-hydroxybutyrate)-Poly(ethylene glycol); PHB; Bone Marrow Stromal Cells; BMSCs; PEGylation; IN-VITRO; STEM-CELLS; SCAFFOLDS; BLENDS; BIODEGRADATION; BIOCOMPATIBILITY; CRYSTALLIZATION; DEGRADATION; FABRICATION; MORPHOLOGY;

D O I：

10.1166/jbt.2016.1414

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Poly(3-hydroxybutyrate)-poly(ethylene glycol) (PHB-PEG) copolymer is a novel member of poly-hydroxyalkanoates (PHAs) produced by biotechnological PEGylation with improved biocompatibility and biodegradability. We used the PHB-PEG to produce the porous 3D scaffolds for bone tissue engineering. The PHB-PEG scaffolds were made by gas porous formation technique using ammonium carbonate as a porogen. The 3D-scaffolds on the base of homopolymer poly(3-hydroxybutyrate) (PHB) and its blend with PEG 70:30 w/w (PHB+PEG) were used for comparison. In this study morphology (e.g., porosity), hydrophilicity, thermal properties and protein adsorption of PHB-PEG 3D scaffolds were examined. Bone marrow stromal cells (BMSCs) and 3T3 fibroblast cells were cultured on PHB-PEG 3D scaffolds. Cell viability, growth and adhesion on polymer 3D scaffolds were investigated by XTT test and scanning electron microscopy. The obtained data showed that the PHB-PEG copolymer scaffolds demonstrated lower hydrophobicity and better biocompatibility in comparison with the PHB homopolymer scaffolds and these indicators were not inferior to the PHB+PEG blend. It was also shown that PHB-PEG 3D scaffolds are suitable substrate for cell growth and could be applied for bone tissue engineering.

引用

页码：42 / 52

页数：11

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