MoFap7, a ribosome assembly factor, is required for fungal development and plant colonization of Magnaporthe oryzae

被引:12
作者
Li, Lin [1 ]
Zhu, Xue-Ming [1 ]
Shi, Huan-Bin [2 ]
Feng, Xiao-Xiao [1 ]
Liu, Xiao-Hong [1 ]
Lin, Fu-Cheng [1 ]
机构
[1] Zhejiang Univ, Biotechnol Inst, State Key Lab Rice Biol, Hangzhou, Zhejiang, Peoples R China
[2] China Natl Rice Res Inst, State Key Lab Rice Biol, Hangzhou, Zhejiang, Peoples R China
基金
中国国家自然科学基金;
关键词
MoFap7; ribosome assembly factor; fungal development; virulence; Pmk1; pathway; RICE BLAST FUNGUS; TRANSCRIPTION FACTOR; ADENYLATE KINASE; MAP KINASE; INFECTIOUS GROWTH; PROTEIN-KINASE; CELL INVASION; CDC42; PATHOGENICITY; YEAST;
D O I
10.1080/21505594.2019.1697123
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Fap7, an important ribosome assembly factor, plays a vital role in pre-40S small ribosomal subunit synthesis in Saccharomyces cerevisiae via its ATPase activity. Currently, the biological functions of its homologs in filamentous fungi remain elusive. Here, MoFap7, a homologous protein of ScFap7, was identified in the rice blast fungus Magnaporthe oryzae, which is a devastating fungal pathogen in rice and threatens food security worldwide. Delta Mofap7 mutants exhibited defects in growth and development, conidial morphology, appressorium formation and infection, and were sensitive to oxidative stress. In addition, site-directed mutagenesis analysis confirmed that the conserved Walker A motif and Walker B motif in MoFap7 are essential for the biological functions of M. oryzae. We further analyzed the regulation mechanism of MoFap7 in pathogenicity. MoFap7 was found to interact with MoMst50, a regulator functioning in the MAPK Pmk1 signaling pathway, that participates in modulating plant penetration and cell-to-cell invasion by regulating the phosphorylation of MoPmk1. Moreover, MoFap7 interacted with the GTPases MoCdc42 and MoRac1 to control growth and conidiogenesis. Taken together, the results of this study provide novel insights into MoFap7-mediated orchestration of the development and pathogenesis of filamentous fungi.
引用
收藏
页码:1047 / 1063
页数:17
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