Fabrication of Electrochemical Model Influenza A Virus Biosensor Based on the Measurements of Neuroaminidase Enzyme Activity

被引：31

作者：

Anik, Ulku ^{[1
]}

Tepeli, Yudum ^{[1
]}

Diouani, Mohamed F. ^{[2
,3
]}

机构：

[1] Mugla Sitki Kocman Univ, Fac Sci, Dept Chem, TR-48000 Kotekli, Mugla, Turkey

[2] Univ Tunis Elmanar, Inst Pasteur Tunis, LR11 IPT03,PB74,13 Pl Pasteur, Tunis 1002, Tunisia

[3] Univ Carthage, Fac Sci Bizerte, Tunis, Tunisia

来源：

ANALYTICAL CHEMISTRY | 2016年 / 88卷 / 12期

关键词：

HEMAGGLUTININ; IMMUNOSENSOR; H5N1; NEURAMINIDASE; INFECTION; DIAGNOSIS;

D O I：

10.1021/acs.analchem.6b01720

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Neuroaminidase (NA) enzyme is a kind of glycoprotein that is found on the influenza A virus. During infection, NA is important for the release of influenza virions from the host cell surface together with viral aggregates. It may also be involved in targeting the virus to respiratory epithelial cells. In this study, a model electrochemical influenza A viral biosensor in which receptor-binding properties have been based on NA was developed for the first time. The biosensor's working principle is based on monitoring the interactions between fetuin A and NA enzyme. The assay was monitored step by step by using electrochemical impedance spectroscopy.

引用

页码：6151 / 6153

页数：3

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