Separation and Sizing of a Virus Mixture Using Asymmetrical Flow Field-Flow Fractionation Coupled to Multi-Angle Light Scattering

被引：0

作者：

Bou-Assaf, George ^{[1
]}

Blum, Andy ^{[1
]}

Matalka, Omar ^{[1
]}

Frenkel, Ruth ^{[1
]}

Reed, Robert ^{[2
]}

Tadjiki, Soheyl ^{[2
]}

机构：

[1] Biogen, Analyt Dev, Cambridge, MA 02142 USA

[2] Postnova Analyt Inc, Salt Lake City, UT USA

来源：

LC GC EUROPE | 2020年 / 33卷 / 09期

关键词：

VECTOR;

D O I：

暂无

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Biogen and Postnova present data on the analysis of adeno-associated viruses (AAV) using Asymmetrical Flow Field-Flow Fractionation coupled to Multi-Angle Light Scattering detection (AF4-MALS). AAV are promising gene therapy delivery vehicles, whose efficacy may be negatively affected by the presence of viral aggregates. Due to its gentle separation and broad applicable size range, AF4-MALS is able to characterize AAV and their aggregates with high resolution and precision thereby overcoming the drawbacks that column-based chromatography techniques often face when dealing with samples larger than 50 nm in size.

引用

页码：486 / 487

页数：2

共 2 条

[1] Adeno-Associated Virus (AAV) as a Vector for Gene Therapy [J].