A 2D transition metal carbide MXene-based SPR biosensor for ultrasensitive carcinoembryonic antigen detection

被引：185

作者：

Wu, Qiong ^{[1
,2
,3
]}

Li, Ningbo ^{[1
,2
]}

Wang, Ying ^{[1
,2
]}

Liu, Ying ^{[1
,2
]}

Xu, Yanchao ^{[1
,2
]}

Wei, Shuting ^{[1
,2
]}

Wu, Jiandong ^{[1
,2
]}

Jia, Guangri ^{[1
,2
]}

Fang, Xuedong ^{[3
]}

Chen, Fangfang ^{[3
]}

Cui, Xiaoqiang ^{[1
,2
]}

机构：

[1] Jilin Univ, State Key Lab Automot Simulat & Control, Sch Mat Sci & Engn, 2699 Qianjin St, Changchun 130012, Jilin, Peoples R China

[2] Jilin Univ, Key Lab Automobile Mat MOE, 2699 Qianjin St, Changchun 130012, Jilin, Peoples R China

[3] Jilin Univ, China Japan Union Hosp, Dept Gastrointestinal & Colorectal Surg, 126 Sendai St, Changchun 130033, Jilin, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2019年 / 144卷

基金：

中国国家自然科学基金;

关键词：

Surface plasmon resonance (SPR); Carcinoembryonic antigen (CEA); Immunoassay; 2D materials; MXene; SURFACE-PLASMON RESONANCE; CARBON ELECTRODE; GRAPHENE OXIDE; NANOSHEETS; NANOPARTICLES; IMMUNOASSAY; CANCER; NANOCOMPOSITES; PLATFORM; NANOMATERIALS;

D O I：

10.1016/j.bios.2019.111697

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Surface plasmon resonance (SPR) has become a leading technique for in situ bioaffinity assay of diverse targets without need of fluorescent or enzymatic labeling. Nanomaterials-enhanced SPR sensors have developed rapidly and widened the application scope of SPR sensing technology. In this report we describe an ultrasensitive SPR biosensor for detecting carcinoembryonic antigen (CEA). Our SPR biosensor utilizes a Ti3C2-MXene-based sensing platform and multi-walled carbon nanotube (MWCNTs)-polydopamine (PDA)-Ag nanoparticle (AgNPs) signal enhancer. Ti3C2-MXene, a new class of two-dimensional (2D) transition metal carbides, offers a large hydrophilic-biocompatible surface ideal for SPR biosensing. Ti3C2-MXene/AuNPs composites after synthesis are then decorated with staphylococcal protein A (SPA) to orient and immobilize monoclonal anti-CEA antibody (Ab(1)) through its Fc region. By introducing MWCNTs-PDA-AgNPs-polyclonal anti-CEA antibody (MWPAg-Ab(2)) conjugate combined with a sandwich format, the present method provides a dynamic range for CEA determination of 2 x 10(-16) to 2 x 10(-8) M and a detection limit of 0.07 fM. This biosensing approach demonstrates good reproducibility and high specificity for CEA in real serum samples providing a promising method to evaluate CEA in human serum for early diagnosis and monitoring of cancer.

引用

页数：7

共 57 条

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