Simple, Fast, and Sensitive Method for Quantification of Tellurite in Culture Media

被引：30
作者：
Molina, Roberto C.
Burra, Radhika [2 ]
Perez-Donoso, Jose M.
Elias, Alex O.
Munoz, Claudia
Montes, Rebecca A. [2 ]
Chasteen, Thomas G. [2 ]
Vasquez, Claudio C. [1 ]
机构：
[1] Univ Santiago Chile, Mol Microbiol Lab, Dept Biol, Fac Quim & Biol, Santiago, Chile
[2] Sam Houston State Univ, Dept Chem, Huntsville, TX 77340 USA
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2010年 / 76卷 / 14期
关键词：
BIOCHEMICAL-CHARACTERIZATION; RHODOBACTER-CAPSULATUS; REDUCING ACTIVITIES; REDUCTASE-ACTIVITY; ESCHERICHIA-COLI; RESISTANCE;
D O I：
10.1128/AEM.00598-10
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A fast, simple, and reliable chemical method for tellurite quantification is described. The procedure is based on the NaBH(4)-mediated reduction of TeO(3)(2-) followed by the spectrophotometric determination of elemental tellurium in solution. The method is highly reproducible, is stable at different pH values, and exhibits linearity over a broad range of tellurite concentrations.
引用
收藏
页码：4901 / 4904
页数：4
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