A comparison of the influence of material on in vitro cartilage tissue engineering with PCL, PGS, and POC 3D scaffold architecture seeded with chondrocytes

被引:114
作者
Jeong, Claire G. [1 ]
Hollister, Scott J. [1 ,2 ,3 ]
机构
[1] Univ Michigan, Dept Biomed Engn, Ann Arbor, MI 48109 USA
[2] Univ Michigan, Dept Mech Engn, Ann Arbor, MI 48109 USA
[3] Univ Michigan, Dept Surg, Ann Arbor, MI 48109 USA
关键词
Poly (1,8 Octanediol-co-Citrate) (POC); Poly (glycerol sebacate) (PGS); Polycaprolactone (PCL); Chondrogenesis; Controlled scaffold tissue engineering; MESENCHYMAL STEM-CELLS; MATRIX METALLOPROTEINASES; BIODEGRADABLE ELASTOMERS; DEGRADATION; GLYCOSAMINOGLYCANS; CHONDROGENESIS; PROLIFERATION; DESIGN; VIVO;
D O I
10.1016/j.biomaterials.2010.01.145
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
The goal of this study was to determine material effects on cartilage regeneration for scaffolds with the same controlled architecture. The 3D polycaprolactone (PCL), poly (glycerol sebacate) (PGS), and poly (1,8 octanediol-co-citrate) (POC) scaffolds of the same design were physically characterized and tissue regeneration in terms of cell phenotype, cellular proliferation and differentiation, and matrix production were compared to find which material would be most optimal for cartilage regeneration in vitro. POC provided the best support for cartilage regeneration in terms of tissue ingrowth, matrix production, and relative mRNA expressions for chondrocyte differentiation (Col2/Col1). PGS was seen as the least favorable material for cartilage based on its relatively high de-differentiation (Col1), hypertrophic mRNA expression (Col10) and high matrix degradation (MMP13, MMP3) results. PCL still provided microenvironments suitable for cells to be active yet it seemed to cause de-differentiation (Coll) of chondrocytes inside the scaffold while many cells migrated out, growing cartilage outside the scaffold. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码:4304 / 4312
页数:9
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