Yeast proteome map (update 2006)

被引:15
|
作者
Perrot, Michel
Guieysse-Peugeot, Anne-Laure
Massoni, Aurelie
Espagne, Christelle
Claverol, Stephane
Monteiro Silva, Raquel
Jeno, Paul
Santos, Manuel
Bonneu, Marc
Boucherie, Helian
机构
[1] CNRS, IBGC, UMR 5095, F-33077 Bordeaux, France
[2] Univ Bordeaux 2, F-33076 Bordeaux, France
[3] Univ Aveiro, Dept Biol, P-3800 Aveiro, Portugal
[4] Univ Basel, Biozentrum, Dept Biochem, Basel, Switzerland
关键词
proteome; Saccharomyces cerevisiae; yeast;
D O I
10.1002/pmic.200600952
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
To improve the potential of two-dimensional gel electrophoresis for proteomic investigations in yeast we have undertaken the systematic identification of Saccharomyces cerevisiae proteins separated on 2-D gels. We report here the identification of 187 novel protein spots. They were identified by two methods, mass spectrometry and gene inactivation. These identifications extend the number of protein spots identified on our yeast 2-D proteome map to 602, i.e. nearly half the detectable spots of the proteome map. These spots correspond to 417 different proteins. The reference map and the list of identified proteins can be accessed on the Yeast Protein Map server (www.ibgc.u-bordeaux2.fr/YPM).
引用
收藏
页码:1117 / 1120
页数:4
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