Contribution of Chloride Channel Conductance to the Regulation of Resting Membrane Potential in Chondrocytes

被引：26

作者：

Funabashi, Kenji ^{[1
]}

Fujii, Masato ^{[1
]}

Yamamura, Hisao ^{[1
]}

Ohya, Susumu ^{[1
]}

Imaizumi, Yuji ^{[1
]}

机构：

[1] Nagoya City Univ, Dept Mol & Cellular Pharmacol, Grad Sch Pharmaceut Sci, Nagoya, Aichi 4678603, Japan

来源：

JOURNAL OF PHARMACOLOGICAL SCIENCES | 2010年 / 113卷 / 01期

基金：

日本学术振兴会;

关键词：

chondrocyte; chloride channel; niflumic acid; RABBIT ARTICULAR CHONDROCYTES; CA2+-ACTIVATED K+ CHANNEL; CELLS; PROLIFERATION; OUMS-27;

D O I：

10.1254/jphs.10026SC

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The contribution of Cl- conductance relative to that of K+ in the regulation of membrane potential was examined using OUMS-27 cells, a model cell-line of human chondrocytes. Application of 100 mu M niflumic acid (NFA) and other anion-channel blockers induced significant membrane hyperpolarization. The NFA-sensitive membrane current under voltage-clamp was predominantly Cl- current. Application of NFA induced small but significant increase in intracellular Ca2+ concentration ([Ca2+]i) and markedly enhanced the late component of [Ca2+]i rise induced by 1 mu M histamine. In conclusion, Cl- conductance substantially contributes to the regulation of resting membrane potential and [Ca2+]i in OUMS-27 cells.

引用

页码：94 / 99

页数：6

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