Contribution of Chloride Channel Conductance to the Regulation of Resting Membrane Potential in Chondrocytes

被引：25

作者：

Funabashi, Kenji ^{[1
]}

Fujii, Masato ^{[1
]}

Yamamura, Hisao ^{[1
]}

Ohya, Susumu ^{[1
]}

Imaizumi, Yuji ^{[1
]}

机构：

[1] Nagoya City Univ, Dept Mol & Cellular Pharmacol, Grad Sch Pharmaceut Sci, Nagoya, Aichi 4678603, Japan

来源：

JOURNAL OF PHARMACOLOGICAL SCIENCES | 2010年 / 113卷 / 01期

基金：

日本学术振兴会;

关键词：

chondrocyte; chloride channel; niflumic acid; RABBIT ARTICULAR CHONDROCYTES; CA2+-ACTIVATED K+ CHANNEL; CELLS; PROLIFERATION; OUMS-27;

D O I：

10.1254/jphs.10026SC

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The contribution of Cl- conductance relative to that of K+ in the regulation of membrane potential was examined using OUMS-27 cells, a model cell-line of human chondrocytes. Application of 100 mu M niflumic acid (NFA) and other anion-channel blockers induced significant membrane hyperpolarization. The NFA-sensitive membrane current under voltage-clamp was predominantly Cl- current. Application of NFA induced small but significant increase in intracellular Ca2+ concentration ([Ca2+]i) and markedly enhanced the late component of [Ca2+]i rise induced by 1 mu M histamine. In conclusion, Cl- conductance substantially contributes to the regulation of resting membrane potential and [Ca2+]i in OUMS-27 cells.

引用

页码：94 / 99

页数：6

共 15 条

[11] MAST-CELLS, CYTOKINES, AND METALLOPROTEINASES AT THE RHEUMATOID LESION - DUAL IMMUNOLOCALIZATION STUDIES
TETLOW, LC
WOOLLEY, DE
[J]. ANNALS OF THE RHEUMATIC DISEASES, 1995, 54 (11) : 896 - 903
[12] Chloride conductance determining membrane potential of rabbit articular chondrocytes
Tsuga, K
Tohse, N
Yoshino, M
Sugimoto, T
Yamashita, T
Ishii, S
Yabu, H
[J]. JOURNAL OF MEMBRANE BIOLOGY, 2002, 185 (01) : 75 - 81
[13] Observations on the microenvironmental nature of cartilage degradation in rheumatoid arthritis
Woolley, DE
Tetlow, LC
[J]. ANNALS OF THE RHEUMATIC DISEASES, 1997, 56 (03) : 151 - 161
[14] Mechanoregulation of chondrocyte proliferation, maturation, and hypertrophy: Ion-channel dependent transduction of matrix deformation signals
Wu, QQ
Chen, Q
[J]. EXPERIMENTAL CELL RESEARCH, 2000, 256 (02) : 383 - 391
[15] Novel functions of small conductance Ca2+-activated K+ channel in enhanced cell proliferation by ATP in brain endothelial cells
Yamazaki, Daiju
Aoyama, Mineyoshi
Ohya, Susumu
Muraki, Katsuhiko
Asai, Kiyofumi
Imaizumi, Yuji
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (50) : 38430 - 38439

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