Contribution of Chloride Channel Conductance to the Regulation of Resting Membrane Potential in Chondrocytes

被引：25
作者：
Funabashi, Kenji [1 ]
Fujii, Masato [1 ]
Yamamura, Hisao [1 ]
Ohya, Susumu [1 ]
Imaizumi, Yuji [1 ]
机构：
[1] Nagoya City Univ, Dept Mol & Cellular Pharmacol, Grad Sch Pharmaceut Sci, Nagoya, Aichi 4678603, Japan
来源：
JOURNAL OF PHARMACOLOGICAL SCIENCES | 2010年 / 113卷 / 01期
基金：
日本学术振兴会;
关键词：
chondrocyte; chloride channel; niflumic acid; RABBIT ARTICULAR CHONDROCYTES; CA2+-ACTIVATED K+ CHANNEL; CELLS; PROLIFERATION; OUMS-27;
D O I：
10.1254/jphs.10026SC
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
The contribution of Cl- conductance relative to that of K+ in the regulation of membrane potential was examined using OUMS-27 cells, a model cell-line of human chondrocytes. Application of 100 mu M niflumic acid (NFA) and other anion-channel blockers induced significant membrane hyperpolarization. The NFA-sensitive membrane current under voltage-clamp was predominantly Cl- current. Application of NFA induced small but significant increase in intracellular Ca2+ concentration ([Ca2+]i) and markedly enhanced the late component of [Ca2+]i rise induced by 1 mu M histamine. In conclusion, Cl- conductance substantially contributes to the regulation of resting membrane potential and [Ca2+]i in OUMS-27 cells.
引用
收藏
页码：94 / 99
页数：6
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