Contribution of Chloride Channel Conductance to the Regulation of Resting Membrane Potential in Chondrocytes

被引：26

作者：

Funabashi, Kenji ^{[1
]}

Fujii, Masato ^{[1
]}

Yamamura, Hisao ^{[1
]}

Ohya, Susumu ^{[1
]}

Imaizumi, Yuji ^{[1
]}

机构：

[1] Nagoya City Univ, Dept Mol & Cellular Pharmacol, Grad Sch Pharmaceut Sci, Nagoya, Aichi 4678603, Japan

来源：

JOURNAL OF PHARMACOLOGICAL SCIENCES | 2010年 / 113卷 / 01期

基金：

日本学术振兴会;

关键词：

chondrocyte; chloride channel; niflumic acid; RABBIT ARTICULAR CHONDROCYTES; CA2+-ACTIVATED K+ CHANNEL; CELLS; PROLIFERATION; OUMS-27;

D O I：

10.1254/jphs.10026SC

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The contribution of Cl- conductance relative to that of K+ in the regulation of membrane potential was examined using OUMS-27 cells, a model cell-line of human chondrocytes. Application of 100 mu M niflumic acid (NFA) and other anion-channel blockers induced significant membrane hyperpolarization. The NFA-sensitive membrane current under voltage-clamp was predominantly Cl- current. Application of NFA induced small but significant increase in intracellular Ca2+ concentration ([Ca2+]i) and markedly enhanced the late component of [Ca2+]i rise induced by 1 mu M histamine. In conclusion, Cl- conductance substantially contributes to the regulation of resting membrane potential and [Ca2+]i in OUMS-27 cells.

引用

页码：94 / 99

页数：6

共 15 条

[1] Recent developments in the biology and medicinal chemistry of potassium channel modulators: Update from a decade of progress [J].

Coghlan, MJ ;

Carroll, WA ;

Gopalakrishnan, M .

JOURNAL OF MEDICINAL CHEMISTRY, 2001, 44 (11) :1627-1653

[2] ADAMTS-9 is synergistically induced by interleukin-1β and tumor necrosis factor α in OUMS-27 chondrosarcoma cells and in human chondrocytes [J].

Demircan, K ;

Hirohata, S ;

Nishida, K ;

Hatipoglu, OF ;

Oohashi, T ;

Yonezawa, T ;

Apte, SS ;

Ninomiya, Y .

ARTHRITIS AND RHEUMATISM, 2005, 52 (05) :1451-1460

[3] Accelerated Ca2+ entry by membrane hyperpolarization due to Ca2+-activated K+ channel activation in response to histamine in chondrocytes [J].

Funabashi, Kenji ;

Ohya, Susumu ;

Yamamura, Hisao ;

Hatano, Noriyuki ;

Muraki, Katsuhiko ;

Giles, Wayne ;

Imaizumi, Yuji .

AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2010, 298 (04) :C786-C797

[4]

GRISSMER S, 1994, MOL PHARMACOL, V45, P1227

[5] Ryanodine receptor type 2 deficiency changes excitation-contraction coupling and membrane potential in urinary bladder smooth muscle [J].

Hotta, Shingo ;

Morimura, Kozo ;

Ohya, Susumu ;

Muraki, Katsuhiko ;

Takeshima, Hiroshi ;

Imaizumi, Yuji .

JOURNAL OF PHYSIOLOGY-LONDON, 2007, 582 (02) :489-506

[6] Swelling-Activated Cl- Current in Isolated Rabbit Articular Chondrocytes: Inhibition by Arachidonic Acid [J].

Isoya, Eiji ;

Toyoda, Futoshi ;

Imai, Shinji ;

Okumura, Noriaki ;

Kumagai, Kousuke ;

Omatsu-Kanbe, Mariko ;

Kubo, Mitsuhiko ;

Matsuura, Hiroshi ;

Matsusue, Yoshitaka .

JOURNAL OF PHARMACOLOGICAL SCIENCES, 2009, 109 (02) :293-304

[7]

Kunisada T, 1998, INT J CANCER, V77, P854, DOI 10.1002/(SICI)1097-0215(19980911)77:6<854::AID-IJC10>3.0.CO

[8]

2-1

[9] Integrins and stretch activated ion channels; putative components of functional cell surface mechanoreceptors in articular chondrocytes [J].

Mobasheri, A ;

Carter, SD ;

Martín-Vasallo, P ;

Shakibaei, M .

CELL BIOLOGY INTERNATIONAL, 2002, 26 (01) :1-18

[10] Voltage-gated ionic channels in cultured rabbit articular chondrocytes [J].

Sugimoto, T ;

Yoshino, M ;

Nagao, M ;

Ishii, S ;

Yabu, H .

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-PHARMACOLOGY TOXICOLOGY & ENDOCRINOLOGY, 1996, 115 (03) :223-232

← 1 2 →