PM2.5-induced extensive DNA methylation changes in the heart of zebrafish embryos and the protective effect of folic acid

被引：31

作者：

Jiang, Yan ^{[1
,2
]}

Li, Jianxiang ^{[1
,2
]}

Ren, Fei ^{[1
,2
]}

Ji, Cheng ^{[1
]}

Aniagu, Stanley ^{[3
]}

Chen, Tao ^{[1
,2
]}

机构：

[1] Soochow Univ, Med Coll, Suzhou, Peoples R China

[2] Soochow Univ, Jiangsu Key Lab Prevent & Translat Med Geriatr Di, Suzhou, Peoples R China

[3] Texas Commiss Environm Qual, Toxicol Risk Assessment & Res Div, 12015 Pk 35 Cir, Austin, TX USA

来源：

ENVIRONMENTAL POLLUTION | 2019年 / 255卷

基金：

中国国家自然科学基金;

关键词：

PM2.5; Folic acid; DNA methylation; Cardiac developmental toxicity; Zebrafish; CARDIAC DEVELOPMENTAL TOXICITY; AIR-POLLUTION; GENE-EXPRESSION; DANIO-RERIO; EXPOSURE; DISEASE; PM2.5; PREVENTION; DEFECTS; SUPPLEMENTATION;

D O I：

10.1016/j.envpol.2019.113331

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

We previously found that folic acid (FA) attenuated cardiac defects in zebrafish embryos exposed to extractable organic matter (EOM) from PM2.5, but the underlining mechanisms remain to be elucidated. Since DNA methylation is crucial to cardiac development, we hypothesized that EOM-induced aberrant DNA methylation changes could be diminished by FA supplementation. In this study, zebrafish embryos were exposed to EOM in the absence or presence of FA. Genomic-wide DNA methylation analysis identified both DNA hypo- and hyper-methylation changes in CCGG sites in zebrafish embryos exposed to EOM, which were attenuated by FA supplementation. We identified a total of 316 genes with extensive DNA methylation changes in EOM samples but little or no DNA methylation changes in EOM plus FA samples. The genes were involved in critical cellular processes and signaling pathways important for embryo development. In addition, the EOM-decreased SAM/SAH ratio was counteracted by FA supplementation. Furthermore, FA attenuated the EOM-induced changes in the expression of genes involved in the regulation of DNA methylation and in folate biosynthesis. In conclusion, our data suggest that FA supplementation protected zebrafish embryos from the cardiac developmental toxicity of PM2.5 by alleviating EOM-induced DNA methylation changes. (C) 2019 Elsevier Ltd. All rights reserved.

引用

页数：7

共 36 条

[1] Air pollution and congenital heart defects [J].

Agay-Shay, Keren ;

Friger, Michael ;

Linn, Shai ;

Peled, Ammatzia ;

Amitai, Yona ;

Peretz, Chava .

ENVIRONMENTAL RESEARCH, 2013, 124 :28-34

[2] Air pollution and cardiovascular disease - A statement for healthcare professionals from the expert panel on population and prevention science of the American Heart Association [J].

Brook, RD ;

Franklin, B ;

Cascio, W ;

Hong, YL ;

Howard, G ;

Lipsett, M ;

Luepker, R ;

Mittleman, M ;

Samet, J ;

Smith, SC ;

Tager, I .

CIRCULATION, 2004, 109 (21) :2655-2671

[3] Investigation of the effects of folate deficiency on embryonic development through the establishment of a folate deficient mouse model [J].

Burgoon, JM ;

Selhub, J ;

Nadeau, M ;

Sadler, TW .

TERATOLOGY, 2002, 65 (05) :219-227

[4] Folic acid and methionine in the prevention of teratogen-induced congenital defects in mice [J].

Cipollone, Daria ;

Carsetti, Rita ;

Tagliani, Angela ;

Rosado, Maria Manuela ;

Borgiani, Paola ;

Novelli, Giuseppe ;

D'Amati, Giulia ;

Fumagalli, Lorenzo ;

Marino, Bruno ;

Businaro, Rita .

CARDIOVASCULAR PATHOLOGY, 2009, 18 (02) :100-109

[5] TCDD Induced Pericardial Edema and Relative COX-2 Expression in Medaka (Oryzias Latipes) Embryos [J].

Dong, Wu ;

Matsumura, Fumio ;

Kullman, Seth W. .

TOXICOLOGICAL SCIENCES, 2010, 118 (01) :213-223

[6] Multi-organ toxicity induced by fine particulate matter PM2.5 in zebrafish (Danio rerio) model [J].

Duan, Junchao ;

Hu, Hejing ;

Zhang, Yannan ;

Feng, Lin ;

Shi, Yanfeng ;

Miller, Mark R. ;

Sun, Zhiwei .

CHEMOSPHERE, 2017, 180 :24-32

[7] The possible association between exposure to air pollution and the risk for congenital malformations [J].

Farhi, Adel ;

Boyko, Valentina ;

Almagor, Jonatan ;

Benenson, Itzhak ;

Segre, Enrico ;

Rudich, Yinon ;

Stern, Eli ;

Lerner-Geva, Liat .

ENVIRONMENTAL RESEARCH, 2014, 135 :173-180

[8] Folate rescues lithium-, homocysteine- and Wnt3A-induced vertebrate cardiac anomalies [J].

Han, Mingda ;

Serrano, Maria C. ;

Lastra-Vicente, Rosana ;

Brinez, Pilar ;

Acharya, Ganesh ;

Huhta, James C. ;

Chen, Ren ;

Linask, Kersti K. .

DISEASE MODELS & MECHANISMS, 2009, 2 (9-10) :467-478

[9] The Methylcytosine Dioxygenase Tet2 Promotes DNA Demethylation and Activation of Cytokine Gene Expression in T Cells [J].

Ichiyama, Kenji ;

Chen, Tingting ;

Wang, Xiaohu ;

Yan, Xiaowei ;

Kim, Byung-Seok ;

Tanaka, Shinya ;

Ndiaye-Lobry, Delphine ;

Deng, Yuhua ;

Zou, Yanli ;

Zheng, Pan ;

Tian, Qiang ;

Aifantis, Iannis ;

Wei, Lai ;

Dong, Chen .

IMMUNITY, 2015, 42 (04) :613-626

[10] DANA elements: A family of composite, tRNA-derived short interspersed DNA elements associated with mutational activities in zebrafish (Danio rerio) [J].

Izsvak, Z ;

Ivics, Z ;

GarciaEstefania, D ;

Fahrenkrug, SC ;

Hackett, PB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (03) :1077-1081

← 1 2 3 4 →