LC/ESI-MS/MS Method for the Simultaneous Determination of Macrocyclic Lactone Parasiticides in Livestock Products and Fish

被引:6
作者
Inoue, Koichi [1 ]
Yoshimi, Yukiko [1 ]
Hino, Tomoaki [1 ]
Oka, Hisao [1 ,2 ]
机构
[1] Kinjo Gakuin Univ, Sch Pharm, Dept Phys & Analyt Chem, Moriyama Ku, Nagoya, Aichi 4638521, Japan
[2] Kinjo Gakuin Univ, Grad Sch Human Ecol, Moriyama Ku, Nagoya, Aichi 4638521, Japan
来源
FOOD HYGIENE AND SAFETY SCIENCE | 2010年 / 51卷 / 01期
关键词
macrocyclic lactones; abamectin; emamectin; ivermectin; eprinomectin; doramectin; moxidectin; LC/MS/MS; LIQUID-CHROMATOGRAPHY; MASS-SPECTROMETRY; IVERMECTIN; ABAMECTIN; EMAMECTIN; EPRINOMECTIN; AVERMECTINS; MOXIDECTIN; DORAMECTIN; CONFIRMATION;
D O I
10.3358/shokueishi.51.1
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
An LC/ESI (positive-mode)-MS/MS method was developed for simultaneous quantification of 8 macrocyclic lactones (abamectin B1a, abamectin 8,9-Z isomer B1a, emamectin benzoate B1a, emarnectin benzoate 8,9-Z isomer B1a, ivermectin, eprinomectin B1a, doramectin and moxidectin) in animal tissues, egg, milk and honey. The separation was achieved on a TSK-GEL ODS 100 V column (2.0 X 50 mm, 3 mu m) with a mobile phase consisting of 0.1% formic acid in acetonitrile, and 0.1 mM ammonium formate-0.1% formic acid in water, at a flow rate of 0.2 mL/min with gradient elution. Linear calibration plots were obtained with high correlation coefficients (r=0.998-0.999). The LOQ and LOD ranged from 0.02-1.5 ng/mL and 0.1-5 ng/mL, respectively. Average recoveries were in the range of 70.8-117.1% with associated RSD values < 15% (n = 10) for repeatability and reproducibility. The spiking levels for recoveries and RSDs met the validation criteria for Japanese maximum residue limits (MRLs). Based on these results, the proposed analytical method has been proven to be highly efficient and suitable for routine determinations of macrocyclic lactones in animal food matrices.
引用
收藏
页码:1 / 9
页数:9
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