Genotyping-by-Sequencing of the regional Pacific abalone (Haliotis discus) genomes reveals population structures and patterns of gene flow

被引：11

作者：

Nam, Bo-Hye ^{[1
]}

Kim, Hyaekang ^{[2
,3
]}

Seol, Donghyeok ^{[2
,3
,4
]}

Kim, Heebal ^{[2
,3
,4
]}

Noh, Eun Soo ^{[1
]}

Kim, Eun Mi ^{[1
]}

Noh, Jae Koo ^{[1
]}

Kim, Young-Ok ^{[1
]}

Park, Jung Youn ^{[1
]}

Kwak, Woori ^{[4
]}

机构：

[1] Natl Inst Fisheries Sci, Biotechnol Res Div, Busan, South Korea

[2] Seoul Natl Univ, Dept Agr Biotechnol, Seoul, South Korea

[3] Seoul Natl Univ, Res Inst Agr & Life Sci, Seoul, South Korea

[4] Genome Inc, Seoul, South Korea

来源：

PLOS ONE | 2021年 / 16卷 / 04期

关键词：

DEPTH PROTEOMIC ANALYSIS; MICROSATELLITE MARKERS; NATURAL-POPULATIONS; SEA-STAR; HANNAI; MOLLUSK; KOREA; MATRIX; WILD; CONNECTIVITY;

D O I：

10.1371/journal.pone.0247815

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Continuous monitoring of the present genetic status is essential to preserve the genetic resource of wild populations. In this study, we sequenced regional Pacific abalone Haliotis discus samples from three different locations around the Korean peninsula to assess population structure, utilizing Genotyping-by-Sequencing (GBS) method. Using PstI enzyme for genome reduction, we demonstrated the resultant library represented the whole genome region with even spacing, and as a result 16,603 single nucleotide variants (SNVs) were produced. Genetic diversity and population structure were investigated using several methods, and a strong genetic heterogeneity was observed in the Korean abalone populations. Additionally, by comparison of the variant sets among population groups, we were able to discover 26 Korean abalone population-specific SNVs, potentially associated with phenotype differences. This is the first study demonstrating the feasibility of GBS for population genetic study on H. discus. Our results will provide valuable data for the genetic conservation and management of wild abalone populations in Korea and help future GBS studies on the marine mollusks.

引用

页数：17

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