Evaluation of the genetic diversity of pomegranate accessions from Turkey using new microsatellite markers

被引:26
作者
Caliskan, Oguzhan [1 ]
Bayazit, Safder [1 ]
Oktem, Mert [2 ]
Ergul, Ali [3 ]
机构
[1] Mustafa Kemal Univ, Dept Hort, Fac Agr, Antakya, Hatay, Turkey
[2] Mersin Univ, Inst Sci, Dept Biotechnol, Mersin, Turkey
[3] Ankara Univ, Biotechnol Inst, Ankara, Turkey
关键词
Genetic diversity; new microsatellites; pomegranate; synonymous group; PUNICA-GRANATUM L; VITIS-VINIFERA L; MOLECULAR CHARACTERIZATION; RAPD MARKERS; CORUH VALLEY; AFLP MARKERS; SSR MARKERS; GENOTYPES; GERMPLASM; REGION;
D O I
10.3906/tar-1606-124
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
Turkey has many valuable genetic resources for pomegranate; however, there have not been many studies on the identification and characterization of these important genetic resources. New microsatellite markers were used to characterize a set of 78 pomegranate accessions from Turkey. Using six SSR primers, a total of 41 alleles were characterized with an average of 4.6 alleles per locus and mean probability of identity ( PI) value of 0.366. These data indicated a high level of polymorphism in pomegranate germplasm. Five synonymous groups could be detected among 30 accessions. This microsatellite-based key is a first step towards a database for markerassisted identification of pomegranate accessions in Turkey. The present study provides essential information to devise a pomegranate core germplasm collection without duplication of plant material, to sustainably manage pomegranate breeding programs, and to establish conservation strategies for preserving local pomegranate genetic resources.
引用
收藏
页码:142 / 153
页数:12
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