Identification of a novel nuclear speckle-type protein, SPOP

被引：110

作者：

Nagai, Y

Kojima, T

Muro, Y

Hachiya, T

Nishizawa, Y

Wakabayashi, T

Hagiwara, M

机构：

[1] TOKYO MED & DENT UNIV,MED RES INST,DEPT ENDOCRINOL,TOKYO 113,JAPAN

[2] NAGOYA UNIV,SCH MED,DEPT ANAT,NAGOYA,AICHI 466,JAPAN

[3] NAGOYA UNIV,SCH MED,DEPT DERMATOL,NAGOYA,AICHI 466,JAPAN

[4] MED & BIOL LABS CO LTD,INA,SAITAMA 396,JAPAN

来源：

FEBS LETTERS | 1997年 / 418卷 / 1-2期

关键词：

autoantibody; cDNA cloning; nuclear protein; POZ domain;

D O I：

10.1016/S0014-5793(97)01340-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel antigen recognized by serum from a scleroderma patient was identified by expression cloning from the HeLa cell cDNA library, The cloned cDNA encoded a 374-amino acid protein with a relative molecular mass of 47 000 and a predicted amino acid sequence 62.7% identical to the hypothetical protein of Caenorhabditis elegans, T16H12.5. The deduced amino acid sequence had a typical POZ domain and an unidentified region conserved during evolution, No zinc finger or RNA recognition motifs were found in this clone, The 2 kbp mRNA encoding the novel clone SPOP (speckle-type POZ protein) was found to be expressed in all human tissues examined, HA-tagged SPOP, transfected and overexpressed in COS7 cells, exhibited a discrete speckled pattern in the nuclei and was co-localized with the splicing factor, snRNP B'/B. Deletion analysis revealed that both the POZ domain and the evolutionarily conserved region at the amino-terminus are required for the nuclear speckled accumulation of SPOP. (C) 1997 Federation of European Biochemical Societies.

引用

页码：23 / 26

页数：4

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