Cultivation of endothelial cells on adhesive protein-free synthetic polymer gels

被引:79
作者
Chen, YM
Shiraishi, N
Satokawa, H
Kakugo, A
Narita, T
Gong, JP
Osada, Y
Yamamoto, K
Ando, J
机构
[1] Hokkaido Univ, Grad Sch Sci, Kita Ku, Sapporo, Hokkaido 0600810, Japan
[2] Univ Tokyo, Grad Sch Med, Tokyo 1130033, Japan
关键词
hydrogel; biocompatibility; cell proliferation; cell culture; tissue engineering; polymer;
D O I
10.1016/j.biomaterials.2004.11.025
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Various hydrogels without modification by any cell adhesive proteins have been investigated as cell scaffolds. The present study shows that bovine fetal aorta endothelial cells can adhere, spread, proliferate, and reach confluence on poly(acrylic acid), poly(sodium p-styrene sulfonate), and poly(2-acrylamido-2- methyl-l-propanesulfonic sodium) gels, whereas cells reach subconfluence on poly(vinyl alcohol) and poly(meth acrylic acid) gels. The proliferation behavior was sensitive to both hydrogel charge density and crosslinker concentration. The relationship between cell proliferation and zeta potential of gels was discussed. It was found that hydrogels with a negative zeta potential higher than about 20mV facilitates cell proliferation. (c) 2004 Elsevier Ltd. All rights reserved.
引用
收藏
页码:4588 / 4596
页数:9
相关论文
共 29 条
  • [1] THE EFFECT OF FLUID SHEAR-STRESS ON THE MIGRATION AND PROLIFERATION OF CULTURED ENDOTHELIAL-CELLS
    ANDO, J
    NOMURA, H
    KAMIYA, A
    [J]. MICROVASCULAR RESEARCH, 1987, 33 (01) : 62 - 70
  • [2] EFFECT OF EXTRACELLULAR ATP LEVEL ON FLOW-INDUCED CA++ RESPONSE IN CULTURED VASCULAR ENDOTHELIAL-CELLS
    ANDO, J
    OHTSUKA, A
    KORENAGA, R
    KAMIYA, A
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 179 (03) : 1192 - 1199
  • [3] The effect of galectin 1 on 3T3 cell proliferation on chitosan membranes
    Chang, YY
    Chen, SJ
    Liang, HC
    Sung, HW
    Lin, CC
    Huang, RN
    [J]. BIOMATERIALS, 2004, 25 (17) : 3603 - 3611
  • [4] Growth of human endothelial cells on photochemically grafted Gly-Arg-Gly-Asp (GRGD) chitosans
    Chung, TW
    Lu, YF
    Wang, SS
    Lin, YS
    Chu, SH
    [J]. BIOMATERIALS, 2002, 23 (24) : 4803 - 4809
  • [5] The selective modulation of endothelial cell mobility on RGD peptide containing surfaces by YIGSR peptides
    Fittkau, MH
    Zilla, P
    Bezuidenhout, D
    Lutolf, M
    Human, P
    Hubbell, JA
    Davies, N
    [J]. BIOMATERIALS, 2005, 26 (02) : 167 - 174
  • [6] Double-network hydrogels with extremely high mechanical strength
    Gong, JP
    Katsuyama, Y
    Kurokawa, T
    Osada, Y
    [J]. ADVANCED MATERIALS, 2003, 15 (14) : 1155 - +
  • [7] FIBROBLAST CELL-PROLIFERATION ON CHARGED HYDROXYETHYL METHACRYLATE COPOLYMERS
    HATTORI, S
    ANDRADE, JD
    HIBBS, JB
    GREGONIS, DE
    KING, RN
    [J]. JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1985, 104 (01) : 72 - 78
  • [8] Hydrogels for biomedical applications
    Hoffman, Allan S.
    [J]. ADVANCED DRUG DELIVERY REVIEWS, 2012, 64 : 18 - 23
  • [9] CELL BEHAVIOR ON POLYMER SURFACES GRAFTED WITH NONIONIC AND IONIC MONOMERS
    KISHIDA, A
    IWATA, H
    TAMADA, Y
    IKADA, Y
    [J]. BIOMATERIALS, 1991, 12 (08) : 786 - 792
  • [10] Interaction of cells on chargeable functional group gradient surfaces
    Lee, JH
    Lee, JW
    Khang, G
    Lee, HB
    [J]. BIOMATERIALS, 1997, 18 (04) : 351 - 358